The Minnesota Grading System of eye bank eyes for age-related macular degeneration
The Minnesota Grading System of eye bank eyes for age-related macular degeneration
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DOI：
10.1167/iovs.04-0342
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发表时间：
2004-12-01
影响因子：
4.4
通讯作者：
Feng, X
中科院分区：
文献类型：
--
作者：
Olsen, TW;Feng, X
目的.明尼苏达分级系统（MGS）是一种评估人类眼库眼睛并确定年龄相关性黄斑变性（AMD）水平的方法，通过使用来自黄斑相关眼病研究（AREDS）的标准和定义。从明尼苏达狮子会眼库捐赠的眼睛（108对）在睫状体平坦部周向切割，以去除前段。将1000 +/- 2.5-mum红宝石球放置在视神经上作为尺寸参考。通过解剖显微镜拍摄数字化、高分辨率、彩色黄斑照片。从其中一个地球仪上取下神经感觉视网膜。重新拍摄下层视网膜色素上皮，用比例三角形定位中央凹。在黄斑照片上叠加网格，并根据AREDS标准对图像进行分级。对20对进行了双侧解剖，并进行了骨关节炎分级。88个地球仪被分为四个MGS类别之一。20个球形中19个（95%）具有对称等级。MGS提供了一种方法来对眼库眼睛的供体组织进行分级，以符合AREDS分类系统。供体组织可用于随后的分子分析，包括基因组学和蛋白质组学。
PURPOSE. The Minnesota Grading System (MGS) is a method to evaluate human eye bank eyes and determine the level of age-related macular degeneration (AMD), by using criteria and definitions from the Age-Related Eye Disease Study (AREDS).METHODS. Donor eyes ( 108 pairs) from the Minnesota Lions Eye Bank were cut circumferentially at the pars plana to remove the anterior segment. A 1000 +/- 2.5-mum ruby sphere was placed on the optic nerve as a size reference. A digital, high-resolution, color macular photograph was taken through a dissecting microscope. The neurosensory retina was removed from one globe of the pair. The underlying retinal pigment epithelium was rephotographed, localizing the fovea with a proportional triangle. A grid was superimposed in the macular photographs and images were graded according to AREDS criteria. Twenty pairs were dissected bilaterally and graded for symmetry.RESULTS. Eighty-eight globes were graded into one of four MGS categories. Nineteen (95%) of 20 globes had symmetric grades.CONCLUSIONS. The MGS provides a methodology to grade donor tissue from eye bank eyes to correspond to the AREDS classification system. Donor tissue may be used for subsequent molecular analysis, including genomics and proteomics.