Intrauterine administration of autologous peripheral blood mononuclear cells promotes implantation rates in patients with repeated failure of IVF-embryo transfer
Intrauterine administration of autologous peripheral blood mononuclear cells promotes implantation rates in patients with repeated failure of IVF-embryo transfer
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DOI：
10.1093/humrep/del312
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发表时间：
2006-12-01
影响因子：
6.1
通讯作者：
Fujii, S.
中科院分区：
文献类型：
--
作者：
Yoshioka, S.;Fujiwara, H.;Fujii, S.
背景：对于IVF-胚胎移植治疗反复失败的不孕患者，目前尚无有效的治疗方法。由于最近的证据表明，母体免疫细胞的一些群体积极支持胚胎植入，我们已经开发了一种新的方法，使用外周血单核细胞（PBMC）。方法：尽管进行了4次或更多次IVF-胚胎移植但未成功妊娠的患者入组本研究（n = 35，35个周期）。取取卵当天采集患者外周血单个核细胞，用HCG培养48 h。两天后，再次从患者新鲜分离PBMC，与培养的PBMC组合，然后施用至患者的宫腔。在第5天进行囊胚移植，并比较PBMC处理组与未处理组的着床成功率。研究结果：PBMC治疗组的临床妊娠率、着床率和活产率（分别为41.2、23.4和35.3%; n = 17、47和16）显著高于未治疗组（分别为11.1、4.1和5.5%; n = 18、49和18）。结论：宫内注射自体外周血单个核细胞可能是一种有效的方法，以改善反复IVF失败患者的胚胎植入。
BACKGROUND: There are few effective approaches to infertile patients with repeated failure in IVF-embryo transfer therapy. Since recent evidence suggests that some populations of maternal immune cells positively support embryo implantation, we have developed a new approach using peripheral blood mononuclear cells (PBMCs). METHODS: Patients who had not experienced successful pregnancy despite four or more IVF-embryo transfer sessions were enrolled in this study (n = 35, 35 cycles). PBMCs were obtained from patients on the day of oocyte retrieval and were cultured with HCG for 48 h. Two days later, PBMCs were freshly isolated from patients again, combined with cultured PBMC and then administered to the intrauterine cavity of the patients. Blastocyst transfer was performed on day 5, and the success of implantation in the PBMC-treated group was compared with that in the non-treated group. RESULTS: Clinical pregnancy rate, implantation rate and live birth rate in the PBMC-treated group (41.2, 23.4 and 35.3%; n = 17, 47 and 16, respectively) were significantly higher than those in the non-treated group (11.1, 4.1 and 5.5%; n = 18, 49 and 18, respectively). CONCLUSION: Intrauterine administration of autologous PBMC may be an effective approach to improve embryo implantation in patients with repeated IVF failures.