Porcine reproductive and respiratory syndrome virus enters cells through a low pH-dependent endocytic pathway
Porcine reproductive and respiratory syndrome virus enters cells through a low pH-dependent endocytic pathway
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DOI：
10.1016/0168-1702(96)01313-5
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发表时间：
1996-06-01
期刊：
影响因子：
5
通讯作者：
Ackermann, MR
中科院分区：
文献类型：
--
作者：
Kreutz, LC;Ackermann, MR
利用多种药物通过不同的机制干扰细胞内囊泡的pH，研究了猪繁殖与呼吸综合征病毒（PRRSV）进入MARC-145细胞的途径。通过测量病毒RNA复制或感染性病毒的产生来评估病毒进入。氯喹、氯化铵和巴弗洛霉素Al以剂量依赖的方式抑制感染性病毒的RNA复制或产生。这些药物在感染前或感染后不久加入细胞中可抑制病毒复制。此外，氯喹对PRRSV复制的影响在培养基的酸性条件下是可逆的。综上所述，这些结果表明，在病毒进入期间需要低pH。电子显微镜数据显示，病毒颗粒在细胞表面或小泡内的网格蛋白样区所包围。此外，在细胞松弛素B和氧化苯胂存在下，PRRSV感染细胞的数量减少。因此，我们的结论是，PRRSV进入可能发生通过微生物依赖性内吞机制，其中一个低的pH值是必要的适当的病毒脱壳。
The entry pathway of porcine reproductive and respiratory syndrome virus (PRRSV) into MARC-145 cells was investigated using a variety of drugs that interfere with the pH of intracellular vesicles by different mechanisms. Virus entry was assessed by measuring viral RNA replication or production of infectious virus. Chloroquine, ammonium chloride and bafilomycin Al inhibited RNA replication or production of infectious virus in a dose-dependent manner. These drugs inhibited virus replication when added to the cells prior to: at infection or soon after infection. Moreover, the effect of chloroquine on PRRSV replication was reversible under acidic conditions of the media, Taken together, these results indicated that a low pH was required during virus entry. Electron microscopic data showed virus particles at the cell surface or within small vesicles which were circumscribed by a clathrin-like zone. In addition, the number of PRRSV-infected cells was decreased in the presence of cytochalasin B and phenylarsine oxide. Thus, we concluded that PRRSV entry might occur through a microfilament-dependent endocytic mechanism in which a low pH is necessary for proper virus uncoating.