Study of freshly excised brain tissues using terahertz imaging.
Study of freshly excised brain tissues using terahertz imaging.
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DOI：
10.1364/boe.5.002837
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发表时间：
2014-08-01
影响因子：
3.4
通讯作者：
Suh JS
中科院分区：
文献类型：
--
作者：
Oh SJ;Kim SH;Ji YB;Jeong K;Park Y;Yang J;Park DW;Noh SK;Kang SG;Huh YM;Son JH;Suh JS
我们证明了使用反射型太赫兹（THz）成像系统可以清楚地区分新切除的全脑组织中的肿瘤与正常脑组织。肿瘤脑组织的太赫兹二值图像表明，太赫兹图像中的肿瘤边界与可见光图像的边界吻合良好。由于髓鞘在脑组织中的分布不同，灰质和白质区域是可区分的。太赫兹图像与磁共振成像（MRI）结果吻合较好。研究了MRI和苏木精和伊红染色的显微图像，以解释新鲜脑组织和石蜡包埋脑组织太赫兹图像的强度差异。结果表明，脱水后的太赫兹信号与细胞密度相对应。因此，太赫兹成像可作为脑肿瘤无标签实时成像的工具，有助于医生在脑外科手术中确定肿瘤边缘。
We demonstrated that tumors in freshly excised whole brain tissue could be differentiated clearly from normal brain tissue using a reflection-type terahertz (THz) imaging system. THz binary images of brain tissues with tumors indicated that the tumor boundaries in the THz images corresponded well to those in visible images. Grey and white-matter regions were distinguishable owing to the different distribution of myelin in the brain tissue. THz images corresponded closely with magnetic resonance imaging (MRI) results. The MRI and hematoxylin and eosin-stained microscopic images were investigated to account for the intensity differences in the THz images for fresh and paraffin-embedded brain tissue. Our results indicated that the THz signals corresponded to the cell density when water was removed. Thus, THz imaging could be used as a tool for label-free and real-time imaging of brain tumors, which would be helpful for physicians to determine tumor margins during brain surgery.