Peroxidase Mimic Cu-MOF Nanosheet for Highly Sensitive Colorimetric Detection of Cysteine
Peroxidase Mimic Cu-MOF Nanosheet for Highly Sensitive Colorimetric Detection of Cysteine
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DOI：
10.1016/j.molstruc.2022.133593
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发表时间：
2022
影响因子：
3.8
通讯作者：
Jingxuan Qiu
中科院分区：
文献类型：
--
作者：
Dongpo Xu;Shuyan Qi;Yongli Li;Xiuxia Wei;Sihui Gong;Fukang Wang;Yiwen Zhu;Yu Liu;Jingxuan Qiu
• Cu-MOF shows the highly oxidizing ability toward H 2 O 2 -TMB system to have the obviously color change. • Cys with different concentrations can reduce the color of the Cu-MOF, H 2 O 2 and TMB reaction system, the visual detection of Cys can be realized easily through the observable color fading by bare eyes without any instrumentation. In the present work, a simple and sensitive colorimetric sensing strategy based on Cu-MOF nanosheet for the detection of cysteine (Cys) is developed. Cu-MOF can catalyze the color change of the H 2 O 2 -TMB detection system from colorless to blue, and produce an obvious UV absorption peak at 652 nm. In the presence of Cys, it can effectively inhibit the color development of H 2 O 2 -TMB. Under optimal conditions, the absorbance value at 652 nm and the Cys concentration range from 0.2 mM to 2.8 mM show a linear relationship. The detection limit of the sensor is 0.03 mM, and it can effectively distinguish Cys from 9 other amino acids and its metabolites. In addition, we also assessed the Cys content in milk, which has practical significance for detection.
