CAMSAP3 accumulates in the pericentrosomal area and accompanies microtubule release from the centrosome via katanin
CAMSAP3 accumulates in the pericentrosomal area and accompanies microtubule release from the centrosome via katanin
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CAMSAP3 在中心体周围区域积聚，并伴随微管通过剑蛋白从中心体释放
DOI：
10.1242/jcs.198010
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发表时间：
2017-05-15
影响因子：
4
通讯作者：
Meng, Wenxiang
中科院分区：
文献类型：
--
作者：
Dong, Congcong;Xu, Honglin;Meng, Wenxiang
摘要上皮细胞具有顶基轴极性，在吸收、排泄等生理功能中起重要作用。在上皮细胞中，大量的非中心体微管（MT）分散在细胞质中，具有顶端-基底极性，并随着上皮细胞执行不同的功能而重新定向。一些先前的研究发现，非中心体MT在中心体处成核，然后释放并易位到其他地方。然而，详细的过程和分子机制在很大程度上仍然未知。在这项研究中，我们发现Nezha，也称为钙调蛋白调节血影蛋白相关蛋白3（CAMSAP 3），一种非中心体MT负端蛋白，积累在中心体周围区域，并伴随着MT从中心体释放;而CAMSAP 3的缺失阻止MT释放，反而导致MT集中在中心体。进一步的研究表明，CAMSAP 3精确地与动力蛋白和katanin协调，以调节MT的脱离过程。总之，我们的结果表明CAMSAP 3是产生非中心体MT的关键分子。总结：非中心体微管在中心体成核，然后释放;在这里，我们表明，CAMSAP 3协调与动力蛋白和katanin介导这种释放。
ABSTRACT The epithelium has an apico-basal axis polarity that plays an important role in absorption, excretion and other physiological functions. In epithelial cells, a substantial number of non-centrosomal microtubules (MTs) are scattered in the cytoplasm with an apico-basal polarity and reorientate as epithelial cells perform different functions. Several previous studies have found that non-centrosomal MTs are nucleated at the centrosome, and then released and translocated elsewhere. However, the detailed process and molecular mechanism remain largely unknown. In this study, we found that Nezha, also called calmodulin-regulated spectrin-associated protein 3 (CAMSAP3), a non-centrosomal MT minus-end protein, accumulates in the pericentrosomal area and accompanies the release of MTs from the centrosome; whereas depletion of CAMSAP3 prevented MT release and instead caused focusing of MTs at centrosomes. Further studies demonstrated that CAMSAP3 precisely coordinates with dynein and katanin to regulate the MT detachment process. In conclusion, our results indicate that CAMSAP3 is a key molecule for generation of non-centrosomal MTs. Summary: Non-centrosomal microtubules are nucleated at centrosomes and then released; here, we show that CAMSAP3 coordinates with dynein and katanin to mediate this release.