What is critical for plant thermogenesis? Differences in mitochondrial activity and protein expression between thermogenic and non-thermogenic skunk cabbages
What is critical for plant thermogenesis? Differences in mitochondrial activity and protein expression between thermogenic and non-thermogenic skunk cabbages
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DOI：
10.1007/s00425-009-1034-z
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发表时间：
2009-12-01
期刊：
影响因子：
4.3
通讯作者：
Inaba, Takehito
中科院分区：
文献类型：
--
作者：
Ito-Inaba, Yasuko;Hida, Yamato;Inaba, Takehito
花序开花期间的产热作用在几种但不是所有的天南星科植物中都有发现。为了了解什么是关键的产热，我们研究了产热和非产热臭鼬甘蓝（Symplocarpus renifolius和Lysichiton camtschatcensis），这是密切相关的形态和发育之间的差异。线粒体生物发生的关键参数，包括密度，呼吸活性，和蛋白质表达这两个物种之间进行了比较。线粒体密度、呼吸活性和交替氧化酶（AOX）含量。Camtschatcensisspadix的线粒体比S.肾叶线粒体中，解偶联蛋白（UCP）含量较高。AOX和UCP基因在L. Camtschatcensis在肉穗花序、佛焰苞、茎和叶等组织中组成型表达。从L. Camtschatcensis，并通过多重序列比对和系统发育树构建分析了它们的一级结构。AOX和UCP蛋白在结构上与其它产热植物中的同工型有着密切的联系。我们的研究结果表明，线粒体密度，呼吸活性和蛋白质表达，而不是AOX或UCP蛋白的一级结构，可能在植物产热中发挥关键作用。
Thermogenesis during the blooming of inflorescence is found in several but not all aroids. To understand what is critical for thermogenesis, we investigated the difference between thermogenic and non-thermogenic skunk cabbages (Symplocarpus renifolius and Lysichiton camtschatcensis), which are closely related in morphology and phylogeny. Critical parameters of mitochondrial biogenesis, including density, respiratory activity, and protein expression were compared between these two species. Mitochondrial density, respiratory activity, and the amount of alternative oxidase (AOX) in L. camtschatcensis spadix mitochondria were lower than in S. renifolius spadix mitochondria, while the level of uncoupling protein (UCP) was higher. AOX and UCP mRNAs in L. camtschatcensis were constitutively expressed in various tissues, such as the spadix, the spathe, the stalk, and the leaves. cDNA encoding two putative thermogenic proteins, AOX and UCP were isolated from L. camtschatcensis, and their primary structure was analyzed by multiple alignment and phylogenetic tree reconstruction. AOX and UCP protein of two the skunk cabbage species are closely related in structure, compared with other isoforms in thermogenic plants. Our results suggest that mitochondrial density, respiratory activity, and protein expression, rather than the primary structure of AOX or UCP proteins, may play critical roles in thermogenesis in plants.