Removal of estrogenic activity of endocrine-disrupting genistein by ligninolytic enzymes from white rot fungi.
Removal of estrogenic activity of endocrine-disrupting genistein by ligninolytic enzymes from white rot fungi.
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DOI：
10.1016/j.femsle.2005.01.023
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发表时间：
2005-03
影响因子：
2.1
通讯作者：
Yuuki Tamagawa;Hirofumi Hirai;S. Kawai;T. Nishida
中科院分区：
文献类型：
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作者：
Yuuki Tamagawa;Hirofumi Hirai;S. Kawai;T. Nishida
在低氮高碳培养基的木质素分解条件下，用白腐菌Phanerochaete sordidaYK-624处理内分泌干扰金雀异黄素。处理4天后金雀异黄素减少93%，并且在处理过程中检测到木质素分解酶、锰过氧化物酶（MnP）和漆酶的活性，这表明金雀异黄素的消失与白腐真菌胞外产生的木质素分解酶有关。因此，用MnP、漆酶和以1-羟基苯并三唑(HBT)作为介体的漆酶介体系统处理金雀异黄酮。 HPLC 分析表明，用 MnP、漆酶或漆酶-HBT 系统处理 4 小时后，反应混合物中的金雀异黄素几乎完全消失。使用酵母双杂交测定系统，还证实三种酶处理在4小时后完全消除了金雀异黄酮的雌激素活性。这些结果强烈表明木质素分解酶可有效消除金雀异黄酮的雌激素活性。
Endocrine-disrupting genistein was treated with the white rot fungusPhanerochaete sordidaYK-624 under ligninolytic condition with low-nitrogen and high-carbon culture medium. Genistein decreased by 93% after 4 days of treatment and the activities of ligninolytic enzymes, manganese peroxidase (MnP) and laccase, were detected during treatment, thus suggesting that the disappearance of genistein is related to ligninolytic enzymes produced extracellularly by white rot fungi. Therefore, genistein was treated with MnP, laccase, and the laccase-mediator system with 1-hydroxybenzotriazole (HBT) as a mediator. HPLC analysis demonstrated that genistein disappeared almost completely in the reaction mixture after 4 h of treatment with either MnP, laccase, or the laccase–HBT system. Using the yeast two-hybrid assay system, it was also confirmed that three enzymatic treatments completely removed the estrogenic activity of genistein after 4 h. These results strongly suggest that ligninolytic enzymes are effective in removing the estrogenic activity of genistein.