Effect of microfabricated microgroove-surface devices on the morphology of mesenchymal stem cells
Effect of microfabricated microgroove-surface devices on the morphology of mesenchymal stem cells
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DOI：
10.1007/s10544-015-0016-0
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发表时间：
2015-11
影响因子：
2.8
通讯作者：
Xiangkai Zhang;T. Aoyama;T. Yasuda;Makoto Oike;A. Ito;J. Tajino;M. Nagai;R. Fujioka;H. Iijima;S. Yamaguchi;Norihiro Kakinuma;H. Kuroki
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文献类型：
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作者：
Xiangkai Zhang;T. Aoyama;T. Yasuda;Makoto Oike;A. Ito;J. Tajino;M. Nagai;R. Fujioka;H. Iijima;S. Yamaguchi;Norihiro Kakinuma;H. Kuroki
已知与细胞接触的材料表面会影响细胞的形态和功能。为了开发一种适合肌腱工程的表面，我们使用了类似于肌腱细胞微环境的锯齿状微槽表面。本研究旨在探讨具有不同脊角（RAs）、脊长（RLs）、脊宽（RWs）和沟宽（GWs）的微沟槽表面对人骨髓间充质干细胞（MSC）形态的影响。以环烯烃聚合物为材料，采用注射-压缩成型的方法制备了微槽表面盘。其余参数固定，考察了不同角度角（180 ~ 30°）、RLs （5 ~ 500 μm）、RWs （5 ~ 500 μm）和GWs （5 ~ 500 μm）的影响。通过光学显微镜和扫描电镜观察不同RAs、RLs、RWs和GWs对细胞之字形形状的影响。用Phalloidin免疫荧光染色观察细胞骨架的变化。通过光学显微镜观察，MSCs在具有不同脊状和沟槽性质的微沟槽表面上呈锯齿状变化。通过扫描电镜观察，微槽表面的细胞形状发生了变化。Phalloidin免疫荧光染色表明，F-actin不仅在细胞丝状伪足内，而且在细胞体内也改变了取向以符合微凹槽。总之，使用注射-压缩成型微加工的锯齿状微槽表面证明了MSCs改变其形状以适应表面的特性。
The surface of a material that is in contact with cells is known to affect cell morphology and function. To develop an appropriate surface for tendon engineering, we used zigzag microgroove surfaces, which are similar to the tenocyte microenvironment. The purpose of this study was to investigate the effect of microgroove surfaces with different ridge angles (RAs), ridge lengths (RLs), ridge widths (RWs), and groove widths (GWs) on human bone marrow-derived mesenchymal stem cell (MSC) shape. Dishes with microgroove surfaces were fabricated using cyclic olefin polymer by injection-compression molding. The other parameters were fixed, and effects of different RAs (180 – 30 °), RLs (5 – 500 μm), RWs (5 – 500 μm), and GWs (5 – 500 μm) were examined. Changes in the zigzag shape of the cell due to different RAs, RLs, RWs, and GWs were observed by optical microscopy and scanning electron microscopy. Cytoskeletal changes were investigated using Phalloidin immunofluorescence staining. As observed by optical microscopy, MSCs changed to a zigzag shape in response to microgroove surfaces with different ridge and groove properties. . As observed by scanning electron microscopy, the cell shape changed at turns in the microgroove surface. Phalloidin immunofluorescence staining indicated that F-actin, not only in cell filopodia but also inside the cell body, changed orientation to conform to the microgrooves. In conclusion, the use of zigzag microgroove surfaces microfabricated by injection-compression molding demonstrated the property of MSCs to alter their shapes to fit the surface.