Tissue self-organization underlies morphogenesis of the notochord
Tissue self-organization underlies morphogenesis of the notochord
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组织自组织是脊索形态发生的基础
DOI：
10.1098/rstb.2017.0320
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Bagnat, Michel
中科院分区：
文献类型：
--
作者：
Norman, James;Sorrell, Emma L.;Hu, Yi;Siripurapu, Vaishnavi;Garcia, Jamie;Bagwell, Jennifer;Charbonneau, Patrick;Lubkin, Sharon R.;Bagnat, Michel
脊索是一种保守的轴向结构，在脊椎动物中作为胚胎轴伸长的流体静力学支架，随后用于正确的脊柱组装。它由一个充满液体的空泡细胞核心组成，周围是一个被细胞外基质包裹的上皮鞘。在形态发生过程中，液泡化细胞使液泡膨胀并以典型的楼梯图案排列。我们研究了这种模式的起源，发现它可以通过简单的物理原理实现。我们能够使用由硅胶管和吸水聚合物珠组成的物理模型来模拟斑马鱼脊索内空泡细胞的排列。生物的结构和物理模型可以准确地描述的理论开发的填充球和泡沫在圆柱体中。我们的实验与物理模型和数值模拟产生了几个预测的脊索组织的关键特征，我们记录和实验测试斑马鱼。总之，我们的数据表明，脊椎动物脊索的组织是由膨胀的空泡细胞的密度和脊索杆的纵横比。因此，我们得出结论，自我组织的脊椎动物脊索的形态发生的基础。这篇文章的一部分，西奥墨菲会议的问题“力学的发展”。
The notochord is a conserved axial structure that in vertebrates serves as a hydrostatic scaffold for embryonic axis elongation and, later on, for proper spine assembly. It consists of a core of large fluid-filled vacuolated cells surrounded by an epithelial sheath that is encased in extracellular matrix. During morphogenesis, the vacuolated cells inflate their vacuole and arrange in a stereotypical staircase pattern. We investigated the origin of this pattern and found that it can be achieved purely by simple physical principles. We are able to model the arrangement of vacuolated cells within the zebrafish notochord using a physical model composed of silicone tubes and water-absorbing polymer beads. The biological structure and the physical model can be accurately described by the theory developed for the packing of spheres and foams in cylinders. Our experiments with physical models and numerical simulations generated several predictions on key features of notochord organization that we documented and tested experimentally in zebrafish. Altogether, our data reveal that the organization of the vertebrate notochord is governed by the density of the osmotically swelling vacuolated cells and the aspect ratio of the notochord rod. We therefore conclude that self-organization underlies morphogenesis of the vertebrate notochord.This article is part of the Theo Murphy meeting issue on ‘Mechanics of development’.
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影响因子：
--
作者：
K. Kawakami
通讯作者：
K. Kawakami
有序圆柱形泡沫结构中的移动边界
DOI：
--
发表时间：
1997
期刊：
影响因子：
--
作者：
S. Hutzler;D. Weaire;R. Crawford
通讯作者：
R. Crawford
DOI：
--
发表时间：
2012
期刊：
--
影响因子：
--
作者：
Patrick Müller;Katherine W. Rogers;Ben M. Jordan;Joon S. Lee;Drew Robson;Sharad Ramanathan;
通讯作者：
Patrick Müller;Katherine W. Rogers;Ben M. Jordan;Joon S. Lee;Drew Robson;Sharad Ramanathan;
两栖动物胚胎的组织区域和模式调控。
影响因子：
2
作者：
Pate,E
通讯作者：
Pate,E
影响因子：
2.7
作者：
Gray, Ryan S.;Wilm, Thomas P.;Smith, Jeff;Bagnat, Michel;Dale, Rodney M.;Topczewski, Jacek;Johnson, Stephen L.;Solnica-Krezel, Lilianna
通讯作者：
Solnica-Krezel, Lilianna