Selective Chemical Imaging of Static Actin in Live Cells
Selective Chemical Imaging of Static Actin in Live Cells
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DOI：
10.1021/ja211708z
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发表时间：
2012-05-23
影响因子：
15
通讯作者：
Arndt, Hans-Dieter
中科院分区：
文献类型：
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作者：
Milroy, Lech-Gustav;Rizzo, Stefano;Arndt, Hans-Dieter
我们对稳定肌动蛋白的天然产物jasplakinolide和chondraamine C进行了合理设计和优化的类似物表征，在固定的渗透性和非渗透性细胞中使用不同的染料和连接物长度组合实现了高效的肌动蛋白染色，从而突出了天然产品支架的分子柔性程度。对可人工合成的无毒类似物的研究导致了一种强大的细胞渗透性探针的特征，该探针可以选择性地成像活细胞中静态的、长寿命的肌动蛋白细丝与动态的F-肌动蛋白和单体G-肌动蛋白群体，而对快速肌动蛋白动态的干扰可以忽略不计。
We have characterized rationally designed and optimized analogues of the actin-stabilizing natural products jasplakinolide and chondramide C. Efficient actin staining was achieved in fixed permeabilized and non-permeabilized cells using different combinations of dye and linker length, thus highlighting the degree of molecular flexibility of the natural product scaffold. Investigations into synthetically accessible, non-toxic analogues have led to the characterization of a powerful cell-permeable probe to selectively image static, long-lived actin filaments against dynamic F-actin and monomeric G-actin populations in live cells, with negligible disruption of rapid actin dynamics.