Identification and characterization of wheat-wheatgrass translocation lines and localization of barley yellow dwarf virus resistance.
Identification and characterization of wheat-wheatgrass translocation lines and localization of barley yellow dwarf virus resistance.
复制标题
DOI：
10.1139/g00-023
复制
发表时间：
2000-08
期刊：
影响因子：
3.1
通讯作者：
O. Crasta;M. Francki;D. B. Bucholtz;H. Sharma;J. Zhang;R. Wang;H. Ohm;J. Anderson
中科院分区：
文献类型：
--
作者：
O. Crasta;M. Francki;D. B. Bucholtz;H. Sharma;J. Zhang;R. Wang;H. Ohm;J. Anderson
通过远缘杂交将农艺学上重要的性状稳定渐渗到作物植物中通常需要产生和鉴定易位系。然而，鉴定含有易位的品系的低效率是利用有价值的外源染色质衍生性状的显著限制。利用标准表型分析方法对小麦-小麦易位系进行鉴定，结果表明，小麦-小麦易位系的鉴定成功率仅为4%。然而，58%的敏感后代的辐照种子含有中间偃麦草染色体特异性重复序列，这表明γ辐射诱导的易位发生率高。对含有外源染色质的感病系、其抗性姊妹系和其它抗性系的限制性片段长度多态性（RFLP）分析表明，γ射线辐照双单体种子后代中有三分之一以上含有小麦-草易位。基因组原位杂交（GISH）分析选定的线证实，这些是小麦-小麦易位系。利用外源染色体特异性重复序列初步鉴定BYDV感病缺失系，然后对其抗性姊妹系进行RFLP分析，这种方法有效地鉴定了抗性易位系，并将BYDV抗性定位于渗入Th的远端。中间染色体
Stable introgression of agronomically important traits into crop plants through wide crossing often requires the generation and identification of translocation lines. However, the low efficiency of identifying lines containing translocations is a significant limitation in utilizing valuable alien chromatin-derived traits. Selection of putative wheatgrass-wheat translocation lines based on segregation ratios of progeny from gamma-irradiated seed using a standard phenotypic analysis resulted in a low 4% success rate of identifying barley yellow dwarf virus (BYDV) resistant and susceptible translocation lines. However, 58% of the susceptible progeny of this irradiated seed contained a Thinopyrum intermedium chromosome-specific repetitive sequence, which indicated that gamma-irradiation-induced translocations occurred at high rate. Restriction fragment length polymorphism (RFLP) analysis of susceptible lines containing alien chromatin, their resistant sister lines and other resistant lines showed that more than one third of the progeny of gamma-irradiated double monosomic seeds contained wheatgrass-wheat translocations. Genomic in situ hybridization (GISH) analysis of selected lines confirmed that these were wheatgrass-wheat translocation lines. This approach of initially identifying BYDV susceptible deletion lines using an alien chromosome-specific repetitive sequence followed by RFLP analysis of their resistant sister lines efficiently identified resistant translocation lines and localized the BYDV resistance to the distal end of the introgressed Th. intermedium chromosome.