On-PKS Baeyer-Villiger-type O-atom insertion catalyzed by luciferase-like monooxygenase OvmO during olimycin biosynthesis
On-PKS Baeyer-Villiger-type O-atom insertion catalyzed by luciferase-like monooxygenase OvmO during olimycin biosynthesis
复制标题
奥利霉素生物合成过程中荧光素酶样单加氧酶 OvmO 催化的 On-PKS Baeyer-Villiger 型 O 原子插入
DOI：
10.1021/acs.orglett.0c00076
复制
发表时间：
2020
期刊：
影响因子：
5.2
通讯作者：
Ju Jianhua
中科院分区：
文献类型：
--
作者：
Zhang Chunyan;Zhang Huaran;Ju Jianhua
通过诱变和培养基优化，在橄榄链霉菌SCSIO T05中激活了一个沉默的安莎霉素生物合成基因簇（ovm）。theovmO失活突变株产生了一种新的分流产物奥利霉素C（1a），沿着一种次要产物奥利霉素D（1b）。这些线性奥利霉素对应物的产生表明，在奥利霉素A（2）线性聚酮骨架的组装期间，类胰蛋白酶单加氧酶（LLM）OvmO催化PKS上的Baeyer-Villiger型氧化。
A silent ansamycin biosynthetic gene cluster (ovm) was activated inStreptomyces olivaceusSCSIO T05 following mutagenesis and media optimization. A new shunt product, olimycin C (1a) was produced by theovmO-inactivated mutant strain, along with a minor product, olimycin D (1b). The production of these linear olimycin counterparts suggest that luciferase-like monooxygenase (LLM) OvmO catalyzes an on-PKS Baeyer–Villiger-type oxidation during assembly of the olimycin A (2) linear polyketide backbone.