Time-domain and spectral-domain optical coherence tomography in the analysis of brain tumor tissue
Time-domain and spectral-domain optical coherence tomography in the analysis of brain tumor tissue
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DOI：
10.1002/lsm.20353
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发表时间：
2006-07-01
影响因子：
2.4
通讯作者：
Giese, A.
中科院分区：
文献类型：
--
作者：
Boehringer, H. J.;Boller, D.;Giese, A.
简介：由于邻近水肿脑、周围浸润区和实体瘤的固有对比度较低，在切除胶质脑肿瘤期间检测残留肿瘤仍然是一个挑战。因此，神经外科医生对能够促进术中分析切除边缘组织的新技术非常感兴趣。材料和方法：对于小鼠模型中的神经胶质瘤和脑肿瘤和神经系统组织的人活检标本的离体成像，我们使用了中心波长为1，310 nm和15 μ m的相干长度，配备有单模光纤和改进的光学相干断层扫描（OCT）适配器，该适配器包含用于在2.5 cm的工作距离处成像的透镜系统。采用840 nm和930 nm超发光二极管（SLD），中心波长为900 nm的谱域断层扫描仪作为第二成像modelity.Results：时域和谱域相干断层扫描均显示了正常脑、浸润区和小鼠脑胶质瘤实体瘤。平行于光学平面的H&E切片的组织学评估表明，可以检测到小于1毫米的肿瘤区域，并且不仅实体瘤，而且被低密度单个肿瘤细胞侵入的脑也产生不同于正常脑的OCT信号。谱域OCT（SD-OCT）显示了高达1.5-2.0 mm组织深度的肿瘤和正常大脑的显著更详细的微观结构，而时域OCT（TD-OCT）的解释在组织深度> 1.0 mm时是困难的。由于快速扫描时间，SD-OCT数据可以以3D数据图的形式获得，这允许对肿瘤-大脑界面进行多平面分析。与我们在实验性胶质瘤中的发现相似，使用SD-OCT采集的人类神经系统组织的图像显示了正常脑组织的特征信号和肿瘤实质的详细显微结构。结论：实验性胶质瘤和人类脑肿瘤标本的谱域OCT根据显微结构和B扫描信号特征区分实体瘤、弥漫性浸润脑组织和邻近正常脑。结合SD-OCT的快速图像采集速率，该技术具有检测残留肿瘤和指导神经外科肿瘤切除的新型术中成像工具的潜力。
Introduction: Detection of residual tumor during resection of glial brain tumors remains a challenge because of a low inherent contrast of adjacent edematous brain, the surrounding infiltration zone, and the solid tumor. Therefore, new technologies that may facilitate an intraoperative analysis of the tissue at the resection edge are of great interest to neurosurgeons.Materials and Methods: For ex vivo imaging of gliomas in a mouse model and human biopsy specimens of brain tumors and nervous system tissue we have used a time-domain Sirius 713 Tomograph with a central wavelength of 1,310 nm and a coherence length of 15 mu m equipped with a mono mode fiber and a modified optical coherence tomography (OCT) adapter containing a lens system for imaging at a working distance of 2.5 cm. A spectral-domain tomograph using 840 nm and 930 nm superluminescence diodes (SLD) with a central wavelength of 900 nm was used as a second imaging modelity.Results: Both time-domain and spectral-domain coherence tomography delineated normal brain, the infiltration zone and solid tumor in murine intracerebral gliomas. Histological evaluation of H&E sections parallel to the optical plain demonstrated that tumor areas of less than a millimeter could be detected and that not only solid tumor, but also brain invaded by a low-density single tumor cells produced an OCT signal different from normal brain. Spectral-domain OCT (SD-OCT) demonstrated a significantly more detailed microstructure of tumor and normal brain up to a tissue depth of 1.5-2.0 mm, whereas the interpretation of time-domain OCT (TD-OCT) was difficult at a tissue depth > 1.0 mm. Because of rapid scanning times SD-OCT data could be acquired as 3D data maps, which allowed a multi-planar analysis of the tumor to brain interface. Similar to our findings in experimental gliomas, images of human nervous system tissue acquired using SD-OCT showed a characteristic signal of normal brain tissue and a detailed microstructure of tumor parenchyma.Conclusion: Spectral-domain OCT of experimental gliomas and human brain tumor specimens differentiates solid tumor, diffusely invaded brain tissue, and adjacent normal brain based on microstructure and B-scan signal characteristics. In conjunction with the rapid image acquisition rates of SD-OCT, this technology carries the potential of a novel intraoperative imaging tool for the detection of residual tumor and guidance of neurosurgical tumor resections.