Inhibition of mouse acrosome reaction and sperm-zona pellucida binding by anti-human sperm membrane protein 1 antibody
Inhibition of mouse acrosome reaction and sperm-zona pellucida binding by anti-human sperm membrane protein 1 antibody
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DOI：
10.1111/j.1745-7262.2007.00247.x
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发表时间：
2007-01-01
影响因子：
2.9
通讯作者：
Xu, Chen
中科院分区：
文献类型：
--
作者：
Cheng, Guo-Yan;Shi, Jian-Li;Xu, Chen
目的：探讨人精子膜蛋白（hSMP-1）在受精过程中的可能功能。方法：克隆并表达hSMP-1细胞外结构域576 bp cDNA片段。用抗重组hSMP-1 （anti-rhSMP-1）抗体间接免疫荧光染色法确定该蛋白在人和小鼠精子上的定位。对10周龄BALB/c小鼠进行精子顶体反应和精子透明带（ZP）结合试验。结果：成功克隆并表达了重组hSMP-1。该蛋白在人和小鼠精子顶体上的表达受到限制。抗rhsmp -1抗体显著降低了每个卵子结合的平均精子数量。与免疫前对照组相比，抗rhsmp -1治疗后顶体反应率降低（P < 0.05）。结论：抗rhsmp -1抗体抑制小鼠顶体反应和精子- zp结合。
Aim: To investigate the possible functions of human sperm membrane protein (hSMP-1) in the process of fertilization. Methods: A 576-bp cDNA fragment of HSD-1 gene coding for the extracellular domain of hSMP-1 was cloned and expressed. The localization of this protein on human and mouse sperm was determined by indirect immunofluorescent staining by using anti-recombinant hSMP-1 (anti-rhSMP-1) antibodies. Sperm acrosome reaction and sperm-zona pellucida (ZP) binding assay were carried out in 10-week-old BALB/c mice. Results: Recombinant hSMP-1 was successfully cloned and expressed. The expression of the native protein was limited on the acrosome of human and mouse sperm. Treatment of anti-rhSMP-1 antibodies significantly decreased the average number of sperms bound to each egg. Meanwhile, the percentage of acrosome reaction was decreased in comparison to pre-immune control after treatment with anti-rhSMP-1 (P < 0.05). Conclusion: The results suggest that anti-rhSMP-1 antibody inhibited mouse acrosome reaction and sperm-ZP binding.