Assessment and Implications of Scattered Microbeam and Broadbeam Synchrotron Radiation for Bystander Effect Studies
Assessment and Implications of Scattered Microbeam and Broadbeam Synchrotron Radiation for Bystander Effect Studies
复制标题
DOI：
10.1667/rr13720.1
复制
发表时间：
2015-12-01
影响因子：
3.4
通讯作者：
Martin, Olga A.
中科院分区：
文献类型：
--
作者：
Lobachevsky, Pavel;Ivashkevich, Alesia;Martin, Olga A.
由于光束结构明确且可控，同步辐射是研究细胞和动物模型中旁观者效应的绝佳工具。尽管与传统辐射相比，同步加速器辐射在此类研究中具有许多优点，但散射辐射剂量暴露的贡献仍然令人担忧。因此，评估了散射辐射对生物体外模型中同步加速器辐射引起的旁观者效应检测的影响。采用基于放射变色 XRQA2 胶片的剂量测定法来测量距暴露于微束放射治疗和宽束 X 射线辐射的区域不同距离处的培养细胞中散射辐射的吸收剂量。散射辐射的水平取决于距离、目标区域的剂量和光束模式。测量位于相同目标距离的细胞中γ-H2AX焦点的数量，并将其用作生物剂量计来评估吸收剂量。确定了通过物理和生物方法测量的吸收剂量值的相关性。 γ-H2AX 测定成功定量了 10 mGy 范围内的散射辐射及其对观察到的辐射引起的旁观者效应的贡献。 (C) 2015 年放射线研究会
Synchrotron radiation is an excellent tool for investigating bystander effects in cell and animal models because of the well-defined and controllable configuration of the beam. Although synchrotron radiation has many advantages for such studies compared to conventional radiation, the contribution of dose exposure from scattered radiation nevertheless remains a source of concern. Therefore, the influence of scattered radiation on the detection of bystander effects induced by synchrotron radiation in biological in vitro models was evaluated. Radiochromic XRQA2 film-based dosimetry was employed to measure the absorbed dose of scattered radiation in cultured cells at various distances from a field exposed to microbeam radiotherapy and broadbeam X-ray radiation. The level of scattered radiation was dependent on the distance, dose in the target zone and beam mode. The number of gamma-H2AX foci in cells positioned at the same target distances was measured and used as a biodosimeter to evaluate the absorbed dose. A correlation of absorbed dose values measured by the physical and biological methods was identified. The gamma-H2AX assay successfully quantitated the scattered radiation in the range starting from 10 mGy and its contribution to the observed radiation-induced bystander effect. (C) 2015 by Radiation Research Society