A novel electromagnet-triggered pillar valve and its application in immunoassay on a centrifugal platform
A novel electromagnet-triggered pillar valve and its application in immunoassay on a centrifugal platform
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一种新型电磁触发柱阀及其在离心平台免疫分析中的应用
DOI：
10.1039/c9lc00043g
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发表时间：
2019-05-21
期刊：
影响因子：
6.1
通讯作者：
Xu, Youchun
中科院分区：
文献类型：
--
作者：
Chen, Yiqi;Shen, Minjie;Xu, Youchun
圆盘实验室是一个强大的微流体平台，它巧妙地利用离心力，在被动或主动阀的帮助下可控地驱动液体。然而，被动阀主要由旋转速度触发，并且容易受到通道的表面化学性质的影响，而主动阀通常需要复杂的制造或致动过程。提出了一种新型电磁触发主动阀，并将其应用于离心平台上。该阀被称为电磁触发柱（ETP）阀，由金属销和压敏粘合剂（PSA）带组成，并且关闭直到销被电磁体抬起以将PSA带与基板部分分离。作为一个典型的应用，该阀被用来构建用于真菌毒素检测的离心芯片。在一个单元中有四个ETP阀，样品和液体试剂可以顺序释放到反应室中，反应室上点有霉菌毒素结合物，以完成整个免疫测定。该芯片同时检测了黄曲霉毒素B1、赭曲霉毒素A、T-2毒素和玉米赤霉烯酮4种霉菌毒素，检测限均低于国家相关部门规定的允许限值，证明了该主动阀的实用性。
The lab-on-a-disc is a powerful microfluidic platform that skillfully takes advantage of centrifugal force to controllably drive liquids with the assistance of passive or active valves. However, the passive valves are mainly triggered by the rotation speed and can be easily influenced by the surface chemistry of the channel, while the active valves usually require a complicated fabrication or actuation procedure. In this study, a novel active valve that can be easily triggered by an electromagnet was proposed and applied on the centrifugation platform. This valve, named the electromagnet-triggered pillar (ETP) valve, consisted of a metal pin and pressure sensitive adhesive (PSA) tape, and is closed until the pin is lifted up by an electromagnet to partially separate the PSA tape from the substrate. As a typical application, this valve is utilized to construct a centrifugal chip for mycotoxin detection. With four ETP valves in a unit, the sample and liquid reagents can be sequentially released into the reaction chamber that was spotted with mycotoxin conjugates to accomplish the whole immunoassay. Four mycotoxins (aflatoxin B1, ochratoxin A, T-2 toxin, and zearalenone) were simultaneously detected on this chip with limits of detection lower than the permissible limits set by the regulatory agencies of China, demonstrating the practicability of this easy-to-use active valve.