Adult male Xenopus laevis can tolerate months of fasting by catabolizing carbohydrates and lipids
Adult male Xenopus laevis can tolerate months of fasting by catabolizing carbohydrates and lipids
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成年雄性非洲爪蟾可以通过分解碳水化合物和脂质来忍受数月的禁食
DOI：
10.1007/s00360-023-01478-5
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Yamauchi Kiyoshi
中科院分区：
文献类型：
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作者：
Nakajima Ami;Yamaguchi Ryo;Sasazaki Maya;Ishihara Akinori;Yamauchi Kiyoshi
据报道，非洲爪蛙（Xenopus laevis）可以忍受长期禁食而不休眠。然而，该物种在禁食期间获取能量的策略尚不清楚。我们进行了 3 个月和 7 个月的禁食实验来研究maleX的代谢情况。长期禁食期间左眼的变化。我们发现，禁食3个月后，一些血清生化参数，如葡萄糖、甘油三酯、游离脂肪酸以及肝糖原的水平降低，而禁食7个月后，甘油三酯水平降低，脂肪体湿重低于进食组，表明脂质分解代谢开始。此外，禁食 3 个月的动物肝脏中糖异生基因（如 pck1、pck2、g6pc1.1 和 g6pc1.2）的转录水平增加，表明糖异生作用上调。我们的结果提出了maleX.的可能性。通过利用多种能量存储分子，laeviscan 能够比之前报道的耐受更长时间的禁食。进一步研究长期禁食对 X 中从碳水化合物到脂质或氨基酸的代谢转换的影响。需要莱维西斯。
The African clawed frog,Xenopus laevis, has been reported to tolerate long-term fasting without dormancy. However, the strategies for energy acquisition during fasting are unclear in this species. We performed 3- and 7-month fasting experiments to investigate how the metabolism of maleX. laevischanges during long-term fasting. We found that the levels of several serum biochemical parameters, such as glucose, triglycerides, and free fatty acids, as well as liver glycogen were reduced after 3 months of fasting, whereas after 7 months of fasting, triglyceride levels were reduced, and fat body wet weight was lower than that of fed group indicating the onset of lipid catabolism. In addition, transcript levels of gluconeogenic genes, such aspck1,pck2,g6pc1.1, andg6pc1.2, were increased in the livers of animals fasted for 3 months, suggesting upregulation of gluconeogenesis. Our results raise the possibility that maleX. laeviscan tolerate much longer fasting than previously reported by utilizing several energy storage molecules. Further investigation of the effects of prolonged fasting on the metabolic switches from carbohydrates to lipids or amino acids inX. laevisis required.