In Vitro Osteogenic Activity of Rat Bone Marrow Derived Mesenchymal Stem Cells Cultured on Transparent Hydroxyapatite Ceramics
In Vitro Osteogenic Activity of Rat Bone Marrow Derived Mesenchymal Stem Cells Cultured on Transparent Hydroxyapatite Ceramics
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DOI：
10.4028/www.scientific.net/kem.254-256.1055
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发表时间：
2003-12
期刊：
影响因子：
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通讯作者：
N. Kotobuki;Daisuke Kawagoe;H. Fujimori;S. Goto;K. Ioku;H. Ohgushi
中科院分区：
文献类型：
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作者：
N. Kotobuki;Daisuke Kawagoe;H. Fujimori;S. Goto;K. Ioku;H. Ohgushi
直接观察各种材料上的培养细胞有利于评估基本的细胞功能，包括细胞在材料上的附着、铺展、增殖和分化。为此，我们制作了透明的羟基磷灰石（tHA）陶瓷，其中培养的大鼠骨髓间充质干细胞（rMSCs）可以通过相差显微镜在铺板后立即可见。种植的rMSCs在tHA陶瓷上粘附并增殖。此外，在tHA陶瓷上培养的细胞能够在成骨条件下分化为成骨细胞，并且显示出与在组织培养聚苯乙烯皿上培养的细胞相当的成骨活性。结果表明，使用tHA陶瓷可以以时间依赖的方式监测培养的细胞。
Direct observation of cultured cells on various materials has benef it for assessment of fundamental cellular functions including cell attachment, spreading, prol iferation and differentiation on the materials. For this purpose, we made transparent hydroxyapatite (tHA) ceramics, where cultured rat bone marrow-derived mesenchymal stem cells (rMSCs) could be visible by phase contrast microscopy immediately after plating. The seeded rMSCs adhered and proliferated on the tHA ceramics. Furtheremore, the cells cultured on the tHA ceram ics were able to differentiate into osteoblasts under osteogenic conditions and showed comparable osteogenic act ivity to the cells cultured on tissue culture polystyrene dishes. The results suggested tha cultured cells could be monitored in a time dependent manner using the tHA ceramics.