Network Analysis Reveals a Common Host-Pathogen Interaction Pattern in Arabidopsis Immune Responses.
Network Analysis Reveals a Common Host-Pathogen Interaction Pattern in Arabidopsis Immune Responses.
复制标题
网络分析揭示了拟南芥免疫反应中常见的宿主-病原体相互作用模式
DOI：
10.3389/fpls.2017.00893
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发表时间：
2017
影响因子：
5.6
通讯作者：
Zhang Z
中科院分区：
文献类型：
--
作者：
Li H;Zhou Y;Zhang Z
许多植物病原菌向寄主细胞分泌致病效应物，以靶向寄主细胞网络中的重要蛋白质。然而，效应器与宿主细胞网络之间的动态相互作用尚不完全清楚。在这里，通过结合拟南芥蛋白-蛋白相互作用网络、已知的丁香假单胞菌和阿拉伯葡萄孢菌效应物的靶标以及免疫应答中的基因表达谱进行综合网络分析。特别是，我们重点研究了效应靶和差异表达基因(Deg)的特征网络拓扑结构。我们发现，效应者倾向于操纵具有较高介数中心度的关键网络位置。效应器目标，特别是那些作为单个效应器的共同目标的目标，往往在网络中聚集在一起。此外，在感染期间，效应器目标与DEG之间的距离随着时间的推移而增加。与这一观察结果相一致的是，与抗病突变体相比，对病原菌敏感的突变体往往在效应器靶标周围有更多的DEG。我们的结果表明，在细胞网络水平上存在一种常见的植物-病原体相互作用模式，其中病原菌采用强大的局部影响模式来干扰寄主网络中的关键位置，植物通过顺序激活效应器靶标远端的基因来组织深度防御。
Many plant pathogens secrete virulence effectors into host cells to target important proteins in host cellular network. However, the dynamic interactions between effectors and host cellular network have not been fully understood. Here, an integrative network analysis was conducted by combining Arabidopsis thaliana protein–protein interaction network, known targets of Pseudomonas syringae and Hyaloperonospora arabidopsidis effectors, and gene expression profiles in the immune response. In particular, we focused on the characteristic network topology of the effector targets and differentially expressed genes (DEGs). We found that effectors tended to manipulate key network positions with higher betweenness centrality. The effector targets, especially those that are common targets of an individual effector, tended to be clustered together in the network. Moreover, the distances between the effector targets and DEGs increased over time during infection. In line with this observation, pathogen-susceptible mutants tended to have more DEGs surrounding the effector targets compared with resistant mutants. Our results suggest a common plant–pathogen interaction pattern at the cellular network level, where pathogens employ potent local impact mode to interfere with key positions in the host network, and plant organizes an in-depth defense by sequentially activating genes distal to the effector targets.