Human L1 retrotransposition:: cis preference versus trans complementation
Human L1 retrotransposition:: cis preference versus trans complementation
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DOI：
10.1128/mcb.21.4.1429-1439.2001
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发表时间：
2001-02-01
影响因子：
5.3
通讯作者：
Moran, JV
中科院分区：
文献类型：
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作者：
Wei, W;Gilbert, N;Moran, JV
长散布的核元件（LINE或L1）约占人类DNA的17%;然而，大约400，000个L1中只有约60个是移动的。L1编码的蛋白质主要动员编码它们的RNA。在低得多的水平下，L1编码的蛋白质可以在试验中起作用，促进突变型L1和其他细胞mRNA的逆转录转座，产生加工过的假基因。突变型L1 RNA的动员频率为野生型L1的反转录转座频率的0.2 - 0.9%，而细胞RNA的动员频率要低得多（约10%）。0.01至野生型水平的0.05%）。因此，我们得出结论，L1编码的蛋白质表现出深刻的顺式偏好，其编码的RNA。这种机制可以使L1保持逆转录转座能力的存在下，压倒性数量的非功能性L1存在于人类DNA中。
Long interspersed nuclear elements (LINEs or L1s) comprise approximately 17% of human DNA; however, only about 60 of the similar to 400,000 L1s are mobile, Using a retrotransposition assay in cultured human cells, we demonstrate that. L1-encoded proteins predominantly mobilize the RNA that encodes them. At much lower levels, L1-encoded proteins can act in trails to promote retrotransposition of mutant L1s and other cellular mRNAs, creating processed pseudogenes. Mutant L1 RNAs are mobilized at 0.2 to 0.9% of the retrotransposition frequency of wild-type L1s, whereas cellular RNAs are mobilized at much lower frequencies (ca. 0.01 to 0.05% of wild-type levels). Thus, we conclude that L1-encoded proteins demonstrate a profound cis preference for their encoding RNA. This mechanism could enable L1 to remain retrotransposition competent in the presence of the overwhelming number of nonfunctional L1s present in human DNA.