Catecholamines and metabolism of human adipose tissue. I. Comparison between in vitro effects of noradrenaline, adrenaline and theophylline on lipolysis in omental adipose tissue.
Catecholamines and metabolism of human adipose tissue. I. Comparison between in vitro effects of noradrenaline, adrenaline and theophylline on lipolysis in omental adipose tissue.
复制标题
儿茶酚胺和人体脂肪组织的代谢。
作者：
J. Ostman;S. Efendić;P. Arner
.本文研究了葡萄糖对离体人网膜脂肪分解的影响。将在全身麻醉期间手术获得的网膜组织在含有3%牛白蛋白的Krebs-Henseleit碳酸氢盐缓冲液中孵育，加入和不加入葡萄糖（2 mg/ml）。甘油释放到培养基中被认为是脂解的指标。葡萄糖可促进基础脂解，并显著增强去甲肾上腺素、异丙基去甲肾上腺素和N6-2′0-二丁酰cAMP（dibutyryl cAMP，DIBAMP）的脂肪动力学作用。甘油释放的斜率获得的二丁酰cAMP在无葡萄糖与含葡萄糖的介质是显着不同的去甲肾上腺素和异丙基去甲肾上腺素。如果承认葡萄糖通过作用于两个不同的位点来刺激脂解，则可以解释这种差异：（a）靠近脂酶裂解酶系统，（B）靠近β-肾上腺素能受体。
在第二个系列的实验中，研究了葡萄糖对FFA水平的影响，在组织中，并在培养基中与去甲肾上腺素存在。未观察到FFA净释放的变化，而证实FFA的组织水平略有增加。人体组织中的这些发现与先前在大鼠附睾脂肪垫中描述的结果不一致，并且不能证明组织FFA水平降低是葡萄糖促进脂解作用的“触发机制”的假设。
当2-脱氧-D-葡萄糖加入到含有去甲肾上腺素、异丙基去甲肾上腺素和二丁酰cAMP的培养基中时，甘油释放量明显减少。这些数据与葡萄糖代谢和甘油三酯脂肪酶活性密切相关的观点一致。
. The influence of glucose on the in vitro lipolysis of human omental adipose tissue has been studied. Omental tissue obtained at surgery during general anesthesia was incubated in Krebs-Henseleit bicarbonate buffer containing 3% bovine albumin with and without added glucose (2 mg/ml). Glycerol release into the medium was considered as an index of lipolysis. Glucose enhanced the basal lipolysis and potentiated significantly the adipokinetic effects of noradrenaline, isopropylnoradrenaline and N6-2′0-dibutyryl cAMP (dibutyryl cAMP). The slope of glycerol release obtained for dibutyryl cAMP in glucose-free versus glucose-containing medium was significantly different from those obtained for noradrenaline and isopropylnoradrenaline. This difference might be explained if it is admitted that glucose stimulates lipolysis by acting on two different sites: (a) close to the triglyceride-splitting enzyme system, and (b) close to the beta-adrenergic receptor.
In a second series of experiments the effect of glucose on the level of FFA was studied in tissue and in medium with noradrenaline present. No changes in the net release of FFA were observed, whereas a slight increase in the tissue level of FFA was demonstrated. These findings in human tissue are not in accordance with those previously described in rat epididymal fat pad, and do not justify the assumption that a decrease in the tissue level of FFA is the “trigger mechanism” for the lipolysis-promoting effect of glucose.
A marked decrease in the glycerol release was noticed when 2-deoxy-d-glucose was added to medium containing noradrenaline, isopropylnoradrenaline and dibutyryl cAMP. The data are consistent with the view that glucose metabolism and the activity of triglyceride lipase are closely related.