The copper-responsive repressor CopR of Lactococcus lactis is a 'winged helix' protein
The copper-responsive repressor CopR of Lactococcus lactis is a 'winged helix' protein
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DOI：
10.1042/bj20081713
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发表时间：
2009-01-15
影响因子：
4.1
通讯作者：
Solioz, Marc
中科院分区：
文献类型：
--
作者：
Cantini, Francesca;Banci, Lucia;Solioz, Marc
乳酸乳球菌的CopR是一种参与铜稳态的铜响应阻遏蛋白。它以铜依赖的方式控制总共11个基因（CopR调节子）的表达。在没有铜的情况下，CopR结合到CopR调节子的启动子上。铜使CopR从启动子上释放，从而允许下游基因进行转录。CopR通过其N末端结构域结合到共有序列为TACANNTGTA的“cop盒”上，该序列在厚壁菌门中是保守的。我们已经解析了CopR的N末端DNA结合结构域的核磁共振溶液结构。该蛋白质折叠具有类似调节地衣芽孢杆菌抗生素抗性的BlaI阻遏蛋白的翼状螺旋结构。CopR不同于其他铜响应阻遏蛋白，并且当前的结构代表了一个新的铜调节蛋白家族，我们提议将其称为CopY家族。
CopR of Lactococcus lactis is a copper-responsive repressor involved in copper homoeostasis. It controls the expression of,I total of I I genes, the CopR regulon, in I copper-dependent manner. In the absence of copper, CopR binds to the promoters of the CopR regulon. Copper releases CopR from the promoters, allowing transcription of the downstream genes to proceed. CopR binds through its N-terminal domain to a 'cop box' of consensus TACANNTGTA, which is conserved in Firmicutes. We have solved the NMR solution structure of the N-terminal DNA-binding domain of CopR. The protein fold has it winged helix structure resembling that of the BlaI repressor which regulates antibiotic resistance in Bacillus licheniformis. CopR differs from other copper-responsive repressors, and the present structure represents a novel family of copper regulators, which we propose to call the CopY family.