Cell swelling induced by the permeant molecules urea or glycerol induces immediate high amplitude thyrotropin and prolactin secretion by perifused adenohypophyseal cells.
Cell swelling induced by the permeant molecules urea or glycerol induces immediate high amplitude thyrotropin and prolactin secretion by perifused adenohypophyseal cells.
复制标题
由渗透分子尿素或甘油诱导的细胞肿胀诱导灌注的腺垂体细胞立即高幅度促甲状腺素和催乳素分泌。
DOI：
10.1016/0006-291x(89)92160-8
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发表时间：
1989
影响因子：
3.1
通讯作者：
McAdams,S
中科院分区：
文献类型：
--
作者：
Wang,X;Sato,N;Greer,MA;Greer,SE;McAdams,S
渗透性分子，尿素和甘油，诱发促甲状腺激素和催乳素的分泌爆发时，添加到急性分散的垂体前叶细胞的细胞外介质。尿素或甘油浓度在26 ~ 104 mM之间，两种激素的分泌量均与尿素或甘油浓度成正比（r>0.89，P<0.001）。同等浓度的不渗透分子甘露醇不会诱导分泌。急性TSH和PRL分泌反应TRH，低渗，渗透分子定性难以区分。这些数据支持我们的假设，细胞肿胀和由此产生的质膜扩张是一个强大的激素分泌诱导剂。由于渗透分子的分泌反应没有减少在无钙培养基中含有0.1 mM EGTA，增加Ca 2+跨质膜运输，提高胞浆Ca 2+浓度似乎不参与。
The permeant molecules, urea and glycerol, evoked a prompt secretory burst of TSH and PRL when added to the extracellular medium of acutely dispersed anterior pituitary cells. Secretion of both hormones was proportional to the concentration of urea or glycerol between 26 and 104 mM (r>0.89, P<0.001). Equivalent concentrations of the impermeant molecule, mannitol, did not induce secretion. The acute TSH and PRL secretory responses to TRH, hyposmolarity, and permeant molecules were qualitatively indistinguishable. These data support our hypothesis that cell swelling and resultant plasmalemma expansion is a potent inducer of hormone secretion. Since the secretory response to permeant molecules was not reduced in a Ca2+-free medium containing 0.1 mM EGTA, an increase in Ca2+transport across the plasmalemma to raise cytosol Ca2+concentration does not appear involved.