Utilization of Murine Laparoscopy for Continuous In-Vivo Assessment of the Liver in Multiple Disease Models
Utilization of Murine Laparoscopy for Continuous In-Vivo Assessment of the Liver in Multiple Disease Models
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DOI：
10.1371/journal.pone.0004776
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发表时间：
2009-03-10
期刊：
影响因子：
3.7
通讯作者：
Elinav, Eran
中科院分区：
文献类型：
--
作者：
Shapira, Yami;Katz, Meirav;Elinav, Eran
背景：目前对小鼠肝脏疾病模型的随访策略存在缺陷，无法在组织水平上持续监测疾病进展，并且需要牺牲动物进行组织采样。目的：在这项研究中，我们旨在开发一种安全的重复性工具，通过利用小型内窥镜系统进行腹腔镜肝脏活检和肿瘤细胞注射到肝脏中。结果：我们报告了一项小鼠腹腔镜检查方案的发展，该方案允许小鼠腹腔内器官的反复可视化。该系统可以在小鼠和大鼠中进行安全且重复的肝脏活检，产生足够的组织进行组织学染色和RNA提取。此外，将肿瘤细胞注射到肝脏中，有利于肝肿瘤在肝脏内的视下植入，从而实现肿瘤生长的可视化。结论：小鼠腹腔镜可作为一种新的影像学方式，用于慢性肝病模型的持续评估和操作。
Background: Current strategies for follow up of murine models of liver disease are flawed by inability to continuously monitor disease progression in the tissue level, and necessitate sacrifice of animals for tissue sampling.Aims: In this study we aimed at developing a safe repetitive tool for sampling livers in vivo, by utilization of a miniaturized endoscopy system for laparoscopic liver biopsies and for injection of tumor cells into livers.Results: We report the development of a protocol for murine laparoscopy that allows repeated visualization of murine intra-abdominal organs. The system enables safe and repeated liver biopsies in mice and rats, yielding adequate tissue for histological staining and RNA extraction. In addition, injection of tumor cells into livers facilitates under-vision implantation of hepatic tumors in liver, followed by visualization of tumor growth.Conclusions: Murine laparoscopy may be employed as a novel imaging modality for continuous assessment and manipulation of chronic liver disease models.