Evaluation of antidepressant activity of methanolic extract of Saraca asoca bark in a chronic unpredictable mild stress model
Evaluation of antidepressant activity of methanolic extract of Saraca asoca bark in a chronic unpredictable mild stress model
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DOI：
10.1097/wnr.0000000000000944
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发表时间：
2018-01-17
期刊：
影响因子：
1.7
通讯作者：
Kumar, Nitesh
中科院分区：
文献类型：
--
作者：
Gill, Meghna;Kinra, Manas;Kumar, Nitesh
长期暴露于应激事件在抑郁症的病因学中起着重要作用。Saraca asoca（Roxb.），De.野生的，或Saraca indica，属于豆科，是印度的内源。花，种子，树皮和植物的叶子已被广泛用于阿育吠陀医学。用S. asoca显示出化学保护、骨髓保护和抗氧化潜力。由于植物的上述特性，本研究试图评估S.使用强迫游泳试验、旷场试验和蔗糖偏好试验，将大鼠每天暴露于慢性不可预测的轻度应激（CIMs），持续8周。还使用过氧化氢酶活性、超氧化物歧化酶活性、还原型谷胱甘肽水平和脑中丙二醛水平评估了提取物对脑中内源性抗氧化剂水平的影响。雄性Sprague-Dawley大鼠在每日应激暴露前1小时每天接受100 mg/kg（口服）提取物，持续8周。与对照组相比，该提取物在强迫游泳试验中显着减少了不动时间，在旷场试验中增加了线交叉，饲养和梳理的总数，并增加了蔗糖消耗以及内源性抗氧化剂的水平显着。因此，S. asoca可能是治疗或减轻抑郁症相关症状的有用药物，可能是通过减少脑中CNS诱导的氧化应激和活性氧物质。
It is well established that chronic exposure to stressful events plays an important role in the etiology of depression. Saraca asoca (Roxb.), De. wild, or Saraca indica, belonging to family Fabaceae, is endogenous to India. The flowers, seeds, bark, and leaves of the plant have been used widely in Ayurveda medicine. The bark extract of S. asoca has shown chemoprotection, myeloprotection, and antioxidant potential. Owing to the above-mentioned properties of the plant, the present study sought to evaluate the effect of a methanolic extract of S. asoca bark in rats exposed to chronic unpredictable mild stress (CUMS) daily for 8 weeks using a forced-swim test, an open-field test, and a sucrose-preference test. The effect of the extract on endogenous antioxidant levels in the brain was also assessed using catalase activity, superoxide dismutase activity, reduced glutathione levels, and malondialdehyde levels in the brain. Male Sprague-Dawley rats received 100mg/kg (oral) of the extract daily 1h before daily stress exposure for 8 weeks. The extract showed a significant reduction in the immobility time in the forced-swim test, increased the total number of line crossing, rearing, and grooming in the open-field test, and increased the sucrose consumption as well as the levels of endogenous antioxidants significantly in comparison with the CUMS control group. Therefore, S. asoca might be a useful agent for the treatment or alleviation of symptoms associated with depression possibly by reducing CUMS-induced oxidative stress and reactive oxygen species in the brain.