Alterations in BDNF and trkB mRNAs following acute or sensitizing cocaine treatments and withdrawal
Alterations in BDNF and trkB mRNAs following acute or sensitizing cocaine treatments and withdrawal
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DOI：
10.1016/j.brainres.2005.11.099
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发表时间：
2006-02-03
期刊：
影响因子：
2.9
通讯作者：
Dziedzicka-Wasylewska, M
中科院分区：
文献类型：
--
作者：
Filip, M;Faron-Górecka, A;Dziedzicka-Wasylewska, M
在本研究中，我们使用原位杂交研究急性或重复可卡因管理和退出重复可卡因治疗对脑源性神经营养因子（BDNF）及其受体trkB mRNA水平的影响在大鼠脑。钴（10 mg/kg i.p.）注射的可卡因急性产生运动过度激活，而重复（单次注射5天）给予可卡因（10 mg/kg）在第10天诱导大鼠响应于激发可卡因剂量（10 mg/kg）的运动活动增加两倍，与盐水处理的动物相比（致敏）。皮质醇处理诱导脑区域特异性降低trkB mRNA水平。另一方面，大鼠海马中的BDNF mRNA仅在可卡因戒断组中增加。可卡因戒断动物表现出显着增加的不动时间测量使用改良的强迫游泳试验。因此，大鼠海马中BDNF mRNA水平的增加似乎与重复可卡因治疗戒断期间的“抑郁样”行为效应相关。在壳（而不是在核心）的核小脑，BDNF mRNA的水平显着增加，急性和重复可卡因治疗以及在可卡因戒断，这表明在大脑区域的神经营养因子水平的变化，可卡因诱导的敏感性的表达涉及其他机制。(C)2005 Elsevier B. V.保留所有权利。
In the present study, we used in situ hybridization to examine the influence of acute or repeated cocaine administrations and withdrawal from repeated cocaine treatment on the level of brain-derived neurotrophic factor (BDNF) and its receptor trkB mRNAs in rat brain. Cocaine (10 mg/kg i.p.) injected acutely produced locomotor hyperactivation, while repeated (single injection for 5 days) administrations of cocaine (10 mg/kg) induced a two-fold increases in the locomotor activity in rats in response to a challenge cocaine dose (10 mg/kg) on day 10, as compared to the saline-treated animals (sensitization). Cocaine treatments induced a brain-region-specific decrease in the levels of trkB mRNA. On the other hand, BDNF mRNA in the rat hippocampus was increased only in the group of rats subjected to cocaine withdrawal. Animals under cocaine withdrawal demonstrated a significant increase in the immobility time measured by the use of modified forced swimming test. Therefore, the increases in the levels of BDNF mRNA in the rat hippocampus seem to be correlated with "depressive-like" behavioral effects during withdrawal from repeated cocaine treatment. In the shell (but not in the core) of the nucleus accumbens, the levels of BDNF mRNA were significantly increased following acute and repeated cocaine treatment as well as during cocaine withdrawal, which indicates that the alterations in the neurotrophin level in the brain region important for the expression of cocaine-induced sensitization involve other mechanisms. (C) 2005 Elsevier B.V. All rights reserved.