A morphometric and immunohistochemical study on angiogenesis of human metastatic carcinomas of the liver
A morphometric and immunohistochemical study on angiogenesis of human metastatic carcinomas of the liver
复制标题
人肝转移癌血管生成的形态测量和免疫组织化学研究
DOI：
10.1002/hep.510240411
复制
发表时间：
1996
期刊：
影响因子：
13.5
通讯作者：
Y. Nakanuma
中科院分区：
文献类型：
--
作者：
Noboru Terayama;T. Terada;Y. Nakanuma
背景/目的：阐明转移性肝癌血管生成过程和肿瘤血管的起源。研究方法：对100例转移癌尸检肝脏进行了血管性血友病因子免疫组化、荆豆凝集素I（UEA‐I）凝集素组织化学和肝转移瘤肿瘤血管密度形态测定研究。此外，在硅橡胶注射的转移性肝脏中三维观察到肿瘤血管。结果：转移性肝癌的肿瘤血管呈血管性血友病因子和UAE‐I凝集素I受体阳性。直径200 μm的肝转移瘤首先出现肿瘤血管。转移瘤内肿瘤血管密度增加，直径达3 mm，直径超过3 mm后血管密度保持稳定。肝转移瘤周围的窦状隙内皮细胞对这些内皮标志物呈阳性。窦状隙内皮细胞常与转移瘤的血管相连。硅橡胶注射的标本显示，周围血窦产生的血管进入转移灶。结论：肝转移瘤的血管生成可随着转移瘤的增大而逐步进行，并可出现肝转移瘤周围血窦内皮毛细血管化。肝转移瘤的肿瘤血管可能部分起源于肿瘤周围的窦状隙内皮细胞。
Background/Aims: To clarify the angiogenetic process and the origin of tumor vessels in human metastatic liver carcinomas. Methods: One hundred autopsy livers with metastatic carcinomas were studied by immunohistochemistry for von Willebrand factor, by lectin histochemistry for Ulex europaeus agglutinin I (UEA‐I), and by morphometry of the density of tumor vessels in the liver metastases. In addition, tumor vessels were observed three‐dimensionally in silicone rubber‐injected livers with metastases. Results: Tumor vessels in metastatic liver carcinomas were positive for von Willebrand factor and UAE‐I agglutinin I receptor. Tumor vessels first appeared in liver metastases of 200 μm in diameter. The density of the tumor vessels in the metastases increased up to 3mm in diameter, and it remained stable over 3mm. Sinusoidal endothelial cells around the liver metastases were positive for these endothelial markers. Sinusoidal endothelial cells were frequently in continuity with vessels in the metastases. Silicone rubber‐injected specimens showed that blood vessels arising from surrounding sinusoids entered into the metastases. Conclusions: The angiogenesis of liver metastases may progress stepwise as the metastases enlarge, and capillarization of sinusoidal endothelium around the liver metastases may occur. Tumor vessels of liver metastases may in part originate from sinusoidal endothelial cells neighboring the tumors.