Electron beam induced fine virtual electrode for mechanical strain microscopy of living cell
Electron beam induced fine virtual electrode for mechanical strain microscopy of living cell
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用于活细胞机械应变显微镜的电子束诱导精细虚拟电极
DOI：
10.1016/j.snb.2016.06.023
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
and Kunihiko Mabuchi
中科院分区：
文献类型：
--
作者：
Takayuki Hoshino;Hiroki Miyazako;Atsuki Nakayama;Akira Wagatsuma;and Kunihiko Mabuchi
我们已经展示了纳米机械应用，使用物理化学和电化学现象的反向电子束光刻（I-EBL），诱导原位二维（2-D）处理湿样品和活细胞后，EB停止在100 nm厚的SiN膜。入射电子束产生一个虚拟电极，然后在扫描轨迹周围引起电化学和电动力学现象。I-EBL处理在10 mM 3，4-亚乙基二氧噻吩（EDOT）稀释水溶液中在沉积的线和空间图案处具有120 nm的半高全宽（FWHM）分辨率。虚拟电极还产生了一个电动局部排斥力，其分辨率<101 μm，朝向带负电荷的纳米颗粒，EB的2-D扫描允许分散在纯水溶液中的纳米颗粒的2-D驱动。虚拟电极还诱导粘附的纳米颗粒的局部脱离和活细胞在盐水溶液中从SiN膜的局部粘附，这可能是由于动电和部分化学蛋白质变性过程。利用活细胞的局部脱离来研究细胞内弹性应变的时空分布，如机械应变显微镜（MSM），其代表细胞内结构中的机械连接。该MSM应提供细胞中力生成位置的可视化。
We have demonstrated nanomechanical applications using physicochemical and electrochemical phenomena of inverted-electron beam lithography (I-EBL), which inducedin-situtwo-dimensional (2-D) processing on wet samples and a living cell after the EB was stoppted in a 100-nm thick SiN membrane. The incident EB generates a virtual electrode and then this induces electrochemical and electrokinetic phenomena around the scanning trajectory. The I-EBL processing has a 120-nm resolution in full-width-at-half-maximum (FWHM) at the deposited line-and-space pattern in 10 mM 3,4-ethylenedioxythiophene (EDOT) diluted water solution. The virtual electrode also causes a electrokinetic local repulsive force with < ∼ 1 μm resolution toward the negatively charged nanoparticles, and the 2-D scanning of the EB allows 2-D actuation of the nanoparticles dispersed in a pure water solution. The virtual electrode also induces local detachment of adherent nanoparticles and focal adhesion of a living cell from the SiN membrane in a saline solution, probably due to both electrokinetic and partly chemical protein denaturation processes. The local detachment of a living cell is utilized to investigate spatio-temporal distributions of intracellular elastic strain as mechanical strain microscopy (MSM), which represents mechanical connectivity in the intracellular structure. This MSM should provide visualization of the location of the force generation in the cell.