THE ELEVATION OF THE CYTOPLASMIC CALCIUM-IONS IN VASCULAR SMOOTH-MUSCLE CELLS IN SHR - MEASUREMENT OF THE FREE CALCIUM-IONS IN SINGLE LIVING CELLS BY LASER-MICROFLUORO-SPECTROMETRY
THE ELEVATION OF THE CYTOPLASMIC CALCIUM-IONS IN VASCULAR SMOOTH-MUSCLE CELLS IN SHR - MEASUREMENT OF THE FREE CALCIUM-IONS IN SINGLE LIVING CELLS BY LASER-MICROFLUORO-SPECTROMETRY
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DOI：
10.1016/s0006-291x(86)80374-6
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发表时间：
1986-11-26
影响因子：
3.1
通讯作者：
YAZAKI, Y
中科院分区：
文献类型：
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作者：
SUGIYAMA, T;YOSHIZUMI, M;YAZAKI, Y
我们开发了一种精确灵敏的系统，用于测量单个细胞的细胞质游离钙浓度（[Ca2+]i），使用紫外激光和Indo-1。通过这种方法，我们首次成功地测量了单个活血管平滑肌细胞的[Ca2+]i。自发性高血压大鼠[Ca2+]i在第6代培养后升高并维持。然而，[Ca2+]i在Goldblatt高血压大鼠中没有升高。因此，这些结果表明，在自发性高血压大鼠血管平滑肌细胞中维持高[Ca2+]i水平是受遗传调控的，这是高血压的机制之一。
We have developed an accurate and sensitive system for the measurement of cytoplasmic free calcium concentrations ([Ca2+]i) of a single cell by using UV-laser and Indo-1. By this method, we made the first successful measurement of [Ca2+]i of single living vascular smooth muscle cells. [Ca2+]i in spontaneously hypertensive rats was elevated and maintained after the 6th passage culture. However, [Ca2+]i in Goldblatt hypertensive rats was not elevated. Thus, these results suggest that the maintenance of high [Ca2+]i levels of vascular smooth muscle cells in spontaneously hypertensive rats is genetically regulated and that it is one of the mechanisms for hypertension.