The apo-enzyme content of aminotransferases in healthy and diseased domestic animals
The apo-enzyme content of aminotransferases in healthy and diseased domestic animals
复制标题
DOI：
10.1111/j.1939-165x.1998.tb01022.x
复制
发表时间：
1998-01-01
影响因子：
1.2
通讯作者：
Erb, H
中科院分区：
文献类型：
--
作者：
Stokol, T;Erb, H
我们研究了临床正常和患病的犬、猫、马和牛的丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）的载脂蛋白酶含量。在添加和不添加外源性吡哆醛-5 '-磷酸（P5 P）的情况下测量转氨酶活性。猿酶的量表示为包含P5 P的氨基转移酶活性的百分比变化。在所有四个物种中，不含P5 P（全酶活性）的转氨酶测定结果与含P5 P（总酶活性）的结果高度相关（斯皮尔曼等级相关性> 0.980）。临床正常患者的中位脱辅基氨基转移酶百分比为：115只犬为11% ALT和0% AST，50只猫为7% ALT和-5% AST，46匹马为6% AST，50头牛为9% AST。在任何物种中，ape酶的量都没有随着空穴酶活性的增加而增加。与临床正常动物相比，患有影响肝脏、肾脏或肌肉组织疾病的犬、猫和马患者的脱辅基氨基转移酶含量无显著差异。在犬和猫患者中，特定肝病之间的ape酶含量没有显著差异。
We investigated the apo-enzyme content of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in clinically normal and ill canine, feline, equine and bovine patients. Aminotransferase activity was measured with and without the addition of exogenous pyridoxal-5'-phosphate (P5P). The amount of ape-enzyme was expressed as the percentage change in aminotransferase activity with the inclusion of P5P. The results of aminotransferase assays without P5P (holo-enzyme activity) were highly correlated to the results obtained with P5P (total enzyme activity) in all four species (Spearman rank correlations > 0.980). The median apo-aminotransferase percentage in clinically normal patients was: 11% ALT and 0% AST in 115 dogs, 7% ALT and -5% AST in 50 cats, 6% AST in 46 horses and 9% AST in 50 cattle. The amount of ape-enzyme did not increase as hole-enzyme activity increased in any of the species. The apo-aminotransferase content was not significantly different in canine, feline and equine patients with diseases affecting the liver, kidney or musculature compared to clinically normal animals. The ape-enzyme content did not differ significantly between specific liver diseases in canine and feline patients.