HLA-G regulates the invasive properties of JEG-3 choriocarcinoma cells by controlling STAT3 activation
HLA-G regulates the invasive properties of JEG-3 choriocarcinoma cells by controlling STAT3 activation
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DOI：
10.1016/j.placenta.2013.07.070
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发表时间：
2013-11-01
期刊：
影响因子：
3.8
通讯作者：
Li, X.
中科院分区：
文献类型：
--
作者：
Liu, X.;Gu, W.;Li, X.
人类白细胞抗原-G(人类白细胞抗原-G)在滋养层细胞中的表达对胚胎着床的成功起着至关重要的作用，而人类白细胞抗原-G的表达降低可能会导致不良的产科结局。在这项研究中，我们通过RNA干扰沉默了JEG-3细胞中的HLA-G表达，结果在Transwell实验中细胞的侵袭能力显著减弱；然而，没有观察到细胞增殖或凋亡的变化。在人Galectin-1处理的JEG-3细胞中，HLAG的下调抑制了信号转导和转录激活因子3(STAT3)的激活，而HLAG的上调则促进了STAT3的激活和侵袭。最重要的是，白介素6(IL-6)，而不是Galectin-1，被证明以剂量依赖的方式拯救侵袭性缺陷。因此，我们证明，人类白细胞抗原G能够通过影响STAT3的激活来调节JEG-3细胞的侵袭，这可能是子痫前期植入缺陷伴随着人类白细胞抗原G低表达的原因。(C)2013爱思唯尔有限公司。保留所有权利。
The expression of human leucocyte antigen-G (HLA-G) in trophoblasts plays a crucial role in successful embryonic implantation, and reduced HLA-G expression might contribute to adverse obstetric outcomes. In this study, we silenced HLA-G expression using RNA interference in JEG-3 cells, resulting in a notably attenuated invasion capacity of the cells in a Transwell assay; however, no alterations in cell proliferation or apoptosis were observed. The down-regulation of HLA-G dampened the activation of signal transducer and activator of transcription 3 (STAT3), whereas the up-regulation of HLA-G promoted STAT3 activation and invasion in JEG-3 cells treated with human galectin-1. Most importantly, interleukin-6 (IL-6), but not galectin-1, was shown to rescue invasion deficiency in a dose-dependent manner. Thus, we demonstrate that HLA-G is able to regulate JEG-3 cell invasion by influencing STAT3 activation, which may underlie the implantation defects accompanying HLA-G hypo-expression in pre-eclampsia. (C) 2013 Elsevier Ltd. All rights reserved.