Microbial complexes in subgingival plaque
Microbial complexes in subgingival plaque
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DOI：
10.1111/j.1600-051x.1998.tb02419.x
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发表时间：
1998-02-01
影响因子：
6.7
通讯作者：
Kent, RL
中科院分区：
文献类型：
--
作者：
Socransky, SS;Haffajee, AD;Kent, RL
一段时间以来，已经识别出细菌物种存在于尺寸斑块中的复合物中。本研究的目的是尝试使用来自大量斑块样本以及不同聚类和秩序技术的数据来定义此类社区。在185名受试者（平均年龄51 +/- 16岁）中，（n = 160）或没有（n = 25）牙周炎的185名受试者（平均年龄为51 +/- 16岁），从每颗牙齿的介体方面取下牙菌斑样品。使用整个基因组DNA探针和棋盘DNA-DNA杂交在13,261个斑块样品中确定了40个尺寸分类单元的存在和水平。每次访问时每颗牙齿的6个地点进行临床评估。使用PHI系数和使用平均的未加权链接排序聚类的物种计算了物种对之间的相似性。使用主要组件分析和对应分析进行社区任命。使用任何分析方法始终观察到5个主要复合物。一种复合物由紧密相关的组组成：菌虫的脚囊，牙龈卟啉单胞菌和treponema denticola。第二络合物由一个紧密相关的核心组组成，包括核细菌核细菌/牙周种的成员，Prevotella Intermedia，Prevotella nigrescens和peptoptostoctoccus micros。与该组相关的物种包括：nodatum菌，弯曲杆菌，弯曲杆菌，弯曲杆菌，链球菌星链球菌和弯曲杆菌。第三络合物由Sanguis链球菌，S。Oralis，S。Mitis，S。Gordonii和S. intermedius组成。第四络合物由3种c经肉芽细胞，弯曲杆菌，弯曲杆菌，二烯菌腐蚀和放线杆菌静脉菌群血清型A组成。第五络合物由Veillonella parvula和放线菌ODONTOLLYTICUS组成。 A.放线症血清型B，Selenomonas noxia和放线菌Naeslundii Genospecies 2（A. viscosus）是离群值，彼此之间几乎没有关系和5个主要复合物。第一个复合物与牙周疾病的临床量度有关，尤其是口袋深度和探测时出血。
It has been recognized for some time that bacterial species exist in complexes in subgingival plaque. The purpose of the present investigation was to attempt to define such communities using data from large numbers of plaque samples and different clustering and ordination techniques. Subgingival plaque samples were taken from the mesial aspect of each tooth in 185 subjects (mean age 51 +/- 16 years) with (n=160) or without (n=25) periodontitis. The presence and levels of 40 subgingival taxa were determined in 13,261 plaque samples using whole genomic DNA probes and checkerboard DNA-DNA hybridization. Clinical assessments were made at 6 sites per tooth at each visit. Similarities between pairs of species were computed using phi coefficients and species clustered using an averaged unweighted linkage sort. Community ordination was performed using principal components analysis and correspondence analysis. 5 major complexes were consistently observed using any of the analytical methods. One complex consisted of the tightly related group: Bacteroides forsythus, Porphyromonas gingivalis and Treponema denticola. The 2nd complex consisted of a tightly related core group including members of the Fusobacterium nucleatum/periodonticum subspecies, Prevotella intermedia, Prevotella nigrescens and Peptostreptococcus micros. Species associated with this group included: Eubacterium nodatum, Campylobacter rectus, Campylobacter showae, Streptococcus constellatus and Campylobacter gracilis. The 3rd complex consisted of Streptococcus sanguis, S. oralis, S. mitis, S. gordonii and S. intermedius. The 4th complex was comprised of 3 Capnocytophage species, Campylobacter concisus, Eikenella corrodens and Actinobacillus actinomycetemcomitans serotype a. The 5th complex consisted of Veillonella parvula and Actinomyces odontolyticus. A. actinomycetemcomitans serotype b, Selenomonas noxia and Actinomyces naeslundii genospecies 2 (A. viscosus) were outliers with little relation to each other and the 5 major complexes. The 1st complex related strikingly to clinical measures of periodontal disease particularly pocket depth and bleeding on probing.