Engineering the Drosophila Genome for Developmental Biology.
Engineering the Drosophila Genome for Developmental Biology.
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DOI：
10.3390/jdb5040016
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发表时间：
2017-12-11
影响因子：
2.7
通讯作者：
Russell S
中科院分区：
文献类型：
--
作者：
Korona D;Koestler SA;Russell S
最近开发的用于体内操纵果蝇基因组的转座子和基于 CRISPR-Cas9 的工具有望在我们研究发育过程的能力方面取得巨大进步。将标签引入基因内源基因组位点的工具有利于细胞或亚细胞水平的成像或生物化学方法。同样，对基因组序列进行特定改变的能力可以实现更精确的遗传控制，以解决基因功能问题。
The recent development of transposon and CRISPR-Cas9-based tools for manipulating the fly genome in vivo promises tremendous progress in our ability to study developmental processes. Tools for introducing tags into genes at their endogenous genomic loci facilitate imaging or biochemistry approaches at the cellular or subcellular levels. Similarly, the ability to make specific alterations to the genome sequence allows much more precise genetic control to address questions of gene function.