Comparative transcriptome analyses of Pseudomonas aeruginosa.
Comparative transcriptome analyses of Pseudomonas aeruginosa.
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DOI：
10.1186/1479-7364-3-4-361
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发表时间：
2009-07
期刊：
影响因子：
4.5
通讯作者：
Mathee K
中科院分区：
文献类型：
--
作者：
Balasubramanian D;Mathee K
细菌生存的标志之一是它们迅速适应不断变化的环境条件的能力。生态位适应是对接收到的信号的反应，这些信号被传递，通常是对调节基因表达的调节器。在后基因组时代，DNA微阵列被用来研究全球范围内基因表达的动态。许多研究已经使用铜绿假单胞菌作为全基因组转录组分析的模式生物。铜绿假单胞菌是一种革兰氏阴性的环境和机会致病菌。本文回顾了转录组的研究，这些研究使我们对这种难治的人类病原体的生物学理解取得了巨大的进步。通过对23个铜绿假单胞菌转录组研究的比较分析，确定了一组独特的信号特异性基因和一组差异调控的核心基因。核心组中的303个基因参与细菌内稳态，使它们成为有吸引力的治疗靶点。
One of the hallmarks of bacterial survival is their ability to adapt rapidly to changing environmental conditions. Niche adaptation is a response to the signals received that are relayed, often to regulators that modulate gene expression. In the post-genomic era, DNA microarrays are used to study the dynamics of gene expression on a global scale. Numerous studies have used Pseudomonas aeruginosa--a Gram-negative environmental and opportunistic human pathogenic bacterium--as the model organism in whole-genome transcriptome analysis. This paper reviews the transcriptome studies that have led to immense advances in our understanding of the biology of this intractable human pathogen. Comparative analysis of 23 P. aeruginosa transcriptome studies has led to the identification of a unique set of genes that are signal specific and a core set that is differentially regulated. The 303 genes in the core set are involved in bacterial homeostasis, making them attractive therapeutic targets.