Wood-mimetic skins prepared using horseradish peroxidase catalysis to induce surface wrinkling of chitosan film upon drying
Wood-mimetic skins prepared using horseradish peroxidase catalysis to induce surface wrinkling of chitosan film upon drying
复制标题
利用辣根过氧化物酶催化诱导壳聚糖膜干燥后表面起皱制备仿木皮
DOI：
10.1016/j.carbpol.2017.06.034
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发表时间：
2017
影响因子：
11.2
通讯作者：
Saimoto Hiroyuki
中科院分区：
文献类型：
--
作者：
Izawa Hironori;Dote Yuki;Okuda Noriko;Sumita Masato;Ifuku Shinsuke;Morimoto Minoru;Saimoto Hiroyuki
我们以前开发的生物基皱纹表面诱导的木材模仿皮肤干燥后，其中微观皱纹上制造的壳聚糖（CS）膜，通过将其浸泡在酚酸溶液中，然后辣根过氧化物酶（HRP）催化的表面反应和干燥。然而，由于分析的困难，所得到的仿木表皮的详细结构，包括交联模式和厚度，还没有被澄清。在这里，我们使用阿魏酸（FE），香草酸（VA）和高香草酸（HO）制备皱纹膜，并表征其结构，以澄清木材仿皮肤的未知特性。用VA和HO制备的仿木皮肤的化学和结构分析表明，在皮肤中的交联结构是由CS和由HRP催化的反应在CS表面上产生的低聚酚残基之间的离子键。此外，这些离子键的数量与皮肤硬度和皱纹大小有关。最后，SEM和TOF-SIMS分析表明，表皮厚度为亚微米级（<200 nm）。
We previously developed bio-based wrinkled surfaces induced by wood-mimetic skins upon drying in which microscopic wrinkles were fabricated on a chitosan (CS) film by immersing it in a phenolic acid solution, followed by horseradish peroxidase (HRP)-catalyzed surface reaction and drying. However, the detailed structure of the resulting wood-mimetic skins, including crosslinking mode and thickness, has not been clarified due to the difficulty of the analysis. Here, we prepare wrinkled films using ferulic acid (FE), vanillic acid (VA), and homovanillic acid (HO) and characterize their structures to clarify the unknown characteristics of wood-mimetic skin. Chemical and structural analyses of wood-mimetic skins prepared using VA and HO indicate that the crosslinking structure in the skin is composed of ionic bonds between CS and an oligophenolic residue generated by the HRP-catalyzed reaction on the CS surface. Moreover, the quantity of these ionic bonds is related to the skin hardness and wrinkle size. Finally, SEM and TOF-SIMS analyses indicate that the skin thickness is on the submicron order (<200 nm).