Identification, rare-event detection and analysis of dendritic cell subsets in bronchoalveolar lavage fluid and peripheral blood by flow cytometry
Identification, rare-event detection and analysis of dendritic cell subsets in bronchoalveolar lavage fluid and peripheral blood by flow cytometry
复制标题
DOI：
10.2741/1185
复制
发表时间：
2003-09-01
期刊：
影响因子：
--
通讯作者：
Donnenberg, AD
中科院分区：
文献类型：
--
作者：
Donnenberg, VS;Donnenberg, AD
树突状细胞(DC)及其前体细胞发挥着重要的作用，不仅作为抗原提呈细胞，而且是免疫调节网络的组成部分。根据其谱系、激活和分化状态的不同，DC可以促进强烈的T细胞反应或无能状态，并可以极化Th1或TH2方向的辅助性T细胞反应。肺是宿主和环境之间的主要界面之一，经常与抗原、过敏原和病原体接触。对这些刺激反应的炎症必须在肺内非常仔细地调节，因为扰动，特别是慢性的扰动，可能会导致免疫病理，干扰肺的关键气体交换功能。尤其是在慢性阻塞性肺疾病(与慢性TH1反应相关)和哮喘(由TH2驱动)等疾病状态下，获取和鉴定DC及其前体的能力对于了解这些过程中的免疫调节至关重要。在这篇报告中，我们将证明成熟的DC及其单核细胞和浆细胞样前体细胞可以通过微创的支气管肺泡灌洗(BAL)程序在肺内采样，尽管它们相对稀少，并可以通过罕见事件多参数流式细胞术进行检测。
Dendritic cells (DCs) and their precursors play important roles, not only as antigen presenting cells, but components of the immunoregulatory network. Depending on their lineage, activation and differentiation state, DCs can promote a strong T-cell response or a state of anergy, and can polarize the T helper response in the direction of TH1 or TH2. The lung comprises one of the major interfaces between the host and the environment and regularly comes into contact with antigens, allergens and pathogens. Inflammation in response to these stimuli must be very carefully regulated in the lung since perturbations, especially of a chronic nature, can result in immunopathology that interferes with the lungs' critical function of gas exchange. Especially in disease states such as chronic obstructive pulmonary disease, which has been associated with a chronic TH1 response, and asthma, which is TH2-driven, the ability to access and characterize DCs and their precursors is critical to the understanding of immune modulation in these processes. In this report we will demonstrate that mature DCs and their monocytoid and plasmacytoid precursors can be sampled in the lung by the minimal invasive procedure of broncho-alveolar lavage (BAL) despite their relative scarcity and can be detected by rare event multiparameter flow cytometry.