RNAseq Analysis Reveals Virus Diversity within Hawaiian Apiary Insect Communities
RNAseq Analysis Reveals Virus Diversity within Hawaiian Apiary Insect Communities
复制标题
DOI：
10.3390/v11050397
复制
发表时间：
2019-05-01
期刊：
影响因子：
4.7
通讯作者：
Martin, Stephen J.
中科院分区：
文献类型：
--
作者：
Brettell, Laura E.;Schroeder, Declan C.;Martin, Stephen J.
变形翅病毒（DWV）是蜜蜂最丰富的病毒病原体，并已与大规模的群体损失。DWV和其他与蜜蜂相关的RNA病毒是能够感染不同宿主的多面手。在这里，我们使用RNAseq分析来检验假设，即由于相互作用的频率，一系列养蜂害虫物种将感染DWV和/或其他蜜蜂相关病毒。我们证实DWV-A是养蜂场中最流行的病毒，在养蜂场害虫中传播的基因序列相似，表明频繁的种间传播。此外，如BLAST分析和基因组覆盖图所示，三种DWV主变体的不同比例揭示了有趣的DWV物种分组。我们还观察到新的基因组重组体由DWV主变体形成，其可能适应于在不同的宿主物种中复制。在考虑其他病毒时，物种分组也适用，其中许多病毒在养蜂场中广泛传播。在社会黄蜂中，样品进一步按位点分组，这也可能影响病毒载量。因此，养蜂场无脊椎动物群落有可能成为蜜蜂相关病毒的储存库，这突出了在考虑蜜蜂健康时考虑养蜂场更广泛的群落的重要性。
Deformed wing virus (DWV) is the most abundant viral pathogen of honey bees and has been associated with large-scale colony losses. DWV and other bee-associated RNA viruses are generalists capable of infecting diverse hosts. Here, we used RNAseq analysis to test the hypothesis that due to the frequency of interactions, a range of apiary pest species would become infected with DWV and/or other honey bee-associated viruses. We confirmed that DWV-A was the most prevalent virus in the apiary, with genetically similar sequences circulating in the apiary pests, suggesting frequent inter-species transmission. In addition, different proportions of the three DWV master variants as indicated by BLAST analysis and genome coverage plots revealed interesting DWV-species groupings. We also observed that new genomic recombinants were formed by the DWV master variants, which are likely adapted to replicate in different host species. Species groupings also applied when considering other viruses, many of which were widespread in the apiaries. In social wasps, samples were grouped further by site, which potentially also influenced viral load. Thus, the apiary invertebrate community has the potential to act as reservoirs of honey bee-associated viruses, highlighting the importance of considering the wider community in the apiary when considering honey bee health.