Transcriptional profile of Pseudomonas syringae pv. phaseolicola NPS3121 in response to tissue extracts from a susceptible Phaseolus vulgaris L. cultivar
Transcriptional profile of Pseudomonas syringae pv. phaseolicola NPS3121 in response to tissue extracts from a susceptible Phaseolus vulgaris L. cultivar
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DOI：
10.1186/1471-2180-9-257
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发表时间：
2009-12-14
期刊：
影响因子：
4.2
通讯作者：
Alvarez-Morales, Ariel
中科院分区：
文献类型：
--
作者：
Hernandez-Morales, Alejandro;De la Torre-Zavala, Susana;Alvarez-Morales, Ariel
背景：假单胞菌pv.菜豆生菌（Phaseolicola）是引起菜豆（Phaseolusvulgaris L.）的“晕枯萎病”的革兰氏阴性植物病原性细菌。这种疾病影响叶子和豆荚，是世界温带地区的主要问题。虽然已经确定了几个细菌基因参与发病机制，但对整个过程仍然知之甚少，主要是因为许多基因的身份和功能在很大程度上是未知的。在这项工作中，一个基因组文库的P.构建菜豆NPS 3121，并进行单个片段的PCR扩增以打印DNA微阵列。结果：转录谱分析表明，在菜豆叶提取物、豆荚提取物和质外体液的作用下，有224个基因表达差异，其中大部分基因在菜豆叶提取物和质外体液的作用下表达差异。一些诱导基因先前已知参与细菌-植物相互作用和毒力的第一阶段。这些包括编码III型分泌系统蛋白的基因和参与细胞壁降解、菜豆毒素合成和有氧代谢的基因。另一方面，大多数被抑制的基因被发现参与铁的吸收和代谢。结论：这项研究进一步了解的机制，反应和代谢适应过程中发生的相互作用的P.与易感寄主植物共生。
Background: Pseudomonas syringae pv. phaseolicola is a Gram-negative plant-pathogenic bacterium that causes "halo blight" disease of beans (Phaseolus vulgaris L.). This disease affects both foliage and pods, and is a major problem in temperate areas of the world. Although several bacterial genes have been determined as participants in pathogenesis, the overall process still remains poorly understood, mainly because the identity and function of many of the genes are largely unknown. In this work, a genomic library of P. syringae pv. phaseolicola NPS3121 was constructed and PCR amplification of individual fragments was carried out in order to print a DNA microarray. This microarray was used to identify genes that are differentially expressed when bean leaf extracts, pod extracts or apoplastic fluid were added to the growth medium.Results: Transcription profiles show that 224 genes were differentially expressed, the majority under the effect of bean leaf extract and apoplastic fluid. Some of the induced genes were previously known to be involved in the first stages of the bacterial-plant interaction and virulence. These include genes encoding type III secretion system proteins and genes involved in cell-wall degradation, phaseolotoxin synthesis and aerobic metabolism. On the other hand, most repressed genes were found to be involved in the uptake and metabolism of iron.Conclusion: This study furthers the understanding of the mechanisms involved, responses and the metabolic adaptation that occurs during the interaction of P. syringae pv. phaseolicola with a susceptible host plant.