Hi-C Analysis in Arabidopsis Identifies the KNOT, a Structure with Similarities to the flamenco Locus of Drosophila
Hi-C Analysis in Arabidopsis Identifies the KNOT, a Structure with Similarities to the flamenco Locus of Drosophila
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DOI：
10.1016/j.molcel.2014.07.009
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发表时间：
2014-09-04
期刊：
影响因子：
16
通讯作者：
Grossniklaus, Ueli
中科院分区：
文献类型：
--
作者：
Grob, Stefan;Schmid, Marc W.;Grossniklaus, Ueli
染色体被折叠，空间组织，并由表观遗传标记调节。染色体结构如何与表观基因组相联系还不清楚。我们发现，拟南芥的染色体结构是紧密相连的表观遗传状态。此外，我们展示了物理约束，如核大小，与染色质的折叠原则。我们还描述了一个核结构，称为结，其中所有五个拟南芥染色体的基因组区域相互作用。这些结接合元件（KEE）区域代表常染色质内的异染色质岛。类似于PIWI相互作用的RNA簇，如果蝇中的弗拉门戈，KEE代表转座因子的首选着陆位点，这可能是拟南芥细胞核中转座子防御机制的一部分。
Chromosomes are folded, spatially organized, and regulated by epigenetic marks. How chromosomal architecture is connected to the epigenome is not well understood. We show that chromosomal architecture of Arabidopsis is tightly linked to the epigenetic state. Furthermore, we show how physical constraints, such as nuclear size, correlate with the folding principles of chromatin. We also describe a nuclear structure, termed KNOT, in which genomic regions of all five Arabidopsis chromosomes interact. These KNOT ENGAGED ELEMENT (KEE) regions represent heterochromatic islands within euchromatin. Similar to PIWI-interacting RNA clusters, such as flamenco in Drosophila, KEEs represent preferred landing sites for transposable elements, which may be part of a transposon defense mechanism in the Arabidopsis nucleus.