Cell viability and drug evaluation biosensing system based on disposable AuNPs/MWCNT nanocomposite modified screen-printed electrode for exocytosis dopamine detection
Cell viability and drug evaluation biosensing system based on disposable AuNPs/MWCNT nanocomposite modified screen-printed electrode for exocytosis dopamine detection
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基于一次性 AuNPs/MWCNT 纳米复合材料修饰丝网印刷电极的细胞活力和药物评价生物传感系统，用于胞吐多巴胺检测
DOI：
10.1016/j.talanta.2022.124118
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发表时间：
2022
期刊：
影响因子：
6.1
通讯作者：
Pin-Fang Huang
中科院分区：
文献类型：
--
作者：
Meng-Meng Liu;Feng-Feng Zhang;Hui Liu;Mei-Juan Wu;Zhou-Jie Liu;Pin-Fang Huang
细胞活力作为评价药物疗效的重要指标，通常采用四唑比色法测定，在药物开发和筛选中起着关键作用。繁琐的操作程序、令人不满意的灵敏度和恶劣的环境困扰着研究人员获取更详细的体内相关生物信息。本文基于多壁碳纳米管、金纳米颗粒和Nafion修饰的丝网印刷电极（SPE）构建了一种简单、低成本的细胞活力和药物评价生物传感系统，用于检测活细胞释放的多巴胺（DA），以评价顺铂和白藜芦醇等药物的细胞毒性。该生物传感系统在复杂生物样品的DA测量中表现出出色的选择性、出色的灵活性和良好的稳定性。此外，在真实的样品中，DA的回收率令人满意，表明该生物传感系统在实际应用中的可靠性和准确性。通过生物传感系统和四氮唑比色法测得的IC 50曲线显示了相似的IC 50值，但剂量-效应关系明显不同，这证实了生物传感器在细胞活力方面的巨大潜力，并描述了细胞功能方面的药效概况。总之，利用固相萃取生物传感器建立的细胞活性和药物评价系统为药物筛选和药物应用开辟了一条新的途径。
Cell viability, as an important index to evaluate drug effects, usually was measured by tetrazolium colorimetric assay, playing a key role in drug development and drug screening. Tedious operating procedures, unsatisfactory sensitivity and abominable environments perplex researchers to acquire more detailedin vivo-relevant biological information. Herein, a simple and low-cost cell viability and drug evaluation biosensing system-based on multiwalled carbon nanotubes, gold nanoparticles and Nafion modified screen-printed electrode (SPE) biosensor was constructed for detection of dopamine (DA) released from living cells to evaluate cytotoxicity of antineoplastic drugs such as cisplatin and resveratrol. The biosensing system was demonstrated to display exceptional selectivity, excellent flexibility and good stability toward DA measurement in complex bio-samples. Additionally, the satisfactory recoveries of DA in real samples revealed the reliability and accuracy of the biosensing system in practical application. The IC50curves respectively obtained by the biosensing system and tetrazolium colorimetric assay provided similar IC50value but distinctly different dose-effect relationship, which confirmed the enormous potential of the biosensor in cell viability and described drug efficacy profiles in cell function. In short, the cell viability and drug evaluation system using SPE biosensor paves a new way in drug screening and pharmaceutical application to measure bioactive molecule such as DA.