Either brain-derived neurotrophic factor or neurotrophin-3 only neurotrophin-producing grafts promote locomotor recovery in untrained spinalized cats.
Either brain-derived neurotrophic factor or neurotrophin-3 only neurotrophin-producing grafts promote locomotor recovery in untrained spinalized cats.
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DOI：
10.1177/1545968314532834
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发表时间：
2015-01
影响因子：
4.2
通讯作者：
Lemay MA
中科院分区：
文献类型：
--
作者：
Ollivier-Lanvin K;Fischer I;Tom V;Houlé JD;Lemay MA
表达脑源性神经营养因子（BDNF）和神经营养因子-3 （NT-3）这两种神经营养因子组合的细胞移植物移植已被证明可以促进和增强未经训练的脊椎猫的运动恢复。基于恢复的时间过程和移植过程中轴突生长的缺失，我们假设恢复是由于神经营养因子介导的腰椎现有运动回路的可塑性。由于BDNF和NT-3对轴突发芽和突触连接/加强有不同的影响，因此确定每种神经营养因子对恢复的贡献变得重要。我们研究了BDNF和NT-3是否能同样有效地恢复未经训练的脊髓猫的运动能力。将分泌两种神经营养因子之一的大鼠成纤维细胞移植到成年猫的T12脊柱横断部位。每组4只猫（单独使用BDNF或单独使用NT-3）进行评估。在横断/移植后3周和5周在跑步机上测试运动恢复情况。两组动物早在3周时就能以高达0.8 m/s的速度行走足底负重，两种移植物在3周和5周时的运动能力相似。即使没有运动训练，BDNF或仅产生NT-3的移植物也能促进完整脊柱动物的运动恢复。需要开发更多临床适用的给药方法。
Transplants of cellular grafts expressing a combination of two neurotrophic factors, brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) have been shown to promote and enhance locomotor recovery in untrained spinalized cats. Based on the time course of recovery and the absence of axonal growth through the transplants, we hypothesized that recovery was due to neurotrophin-mediated plasticity within the existing locomotor circuitry of the lumbar cord. Since BDNF and NT-3 have different effects on axonal sprouting and synaptic connectivity/strengthening, it becomes important to ascertain the contribution of each individual neurotrophins to recovery. We studied whether BDNF or NT-3 only producing cellular grafts would be equally effective at restoring locomotion in untrained spinal cats. Rat fibroblasts secreting one of the two neurotrophins were grafted into the T12 spinal transection site of adult cats. Four cats in each group (BDNF alone or NT-3 alone) were evaluated. Locomotor recovery was tested on a treadmill at 3 and 5 weeks post transection/grafting. Animals in both groups were capable of plantar weight-bearing stepping at speed up to 0.8 m/s as early as 3 weeks and locomotor capabilities were similar at 3 and 5 weeks for both types of graft. Even without locomotor training, either BDNF or NT-3 only producing grafts promote locomotor recovery in complete spinal animals. More clinically applicable delivery methods need to be developed.
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DOI：
10.1111/j.1749-6632.2010.05435.x
发表时间：
2010-01-01
期刊：
NEURONS AND NETWORKS IN THE SPINAL CORD
影响因子：
--
作者：
Markin, Sergey N.;Klishko, Alexander N.;Rybak, Ilya A.
通讯作者：
Rybak, Ilya A.
影响因子：
1.7
作者：
Liu, Y;Himes, BT;Fischer, I
通讯作者：
Fischer, I
影响因子：
2.5
作者：
de Leon, RD;Hodgson, JA;Edgerton, VR
通讯作者：
Edgerton, VR
影响因子：
3.4
作者：
Bradbury, EJ;Khemani, S;McMahon, SB
通讯作者：
McMahon, SB
影响因子：
2.5
作者：
de Leon, RD;Hodgson, JA;Edgerton, VR
通讯作者：
Edgerton, VR