Neutrophil-mediated lung permeability and host defense proteins
Neutrophil-mediated lung permeability and host defense proteins
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DOI：
10.1152/ajplung.00045.2009
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发表时间：
2009-10-01
影响因子：
4.9
通讯作者：
Nelson, Steve
中科院分区：
文献类型：
--
作者：
Kantrow, Stephen P.;Shen, Zhiwei;Nelson, Steve
Kantrow SP，Shen Z，Jagneaux T，Zhang P，Nelson S.中性粒细胞介导的肺通透性和宿主防御蛋白。 Am J Physiol Lung Cell Mol Physiol 297: L738-L745, 2009。首次发表于 2009 年 7 月 31 日； doi:10.1152/ajplung.00045.2009.-中性粒细胞募集到肺泡腔与上皮通透性增加相关。本研究在小鼠中调查了中性粒细胞向肺部募集是否导致血浆衍生的宿主防御蛋白在肺泡腔中积累，以及呼吸爆发是否导致通透性增加。气管内 LPS 激发后 6 小时，支气管肺泡灌洗 (BAL) 液中的白蛋白、补体 C1q 和 IgM 增加。 LPS 处理前的中性粒细胞消耗完全阻止了 BAL 液蛋白质浓度的增加。从 BAL 液中分离出的中性粒细胞中未检测到呼吸爆发，并且与野生型小鼠类似，在缺乏呼吸爆发装置关键亚基 gp91(phox) 的小鼠中，BAL 蛋白增加。气管内滴注趋化因子巨噬细胞炎症蛋白 2 和角质细胞衍生趋化因子引起的中性粒细胞募集也伴随着白蛋白、C1q 和 IgM 的积累。在中性粒细胞募集到肺泡腔期间，上皮通透性促进宿主防御蛋白的递送。观察到的上皮通透性增加需要中性粒细胞的募集，但不需要激活呼吸爆发，并且发生在趋化因子诱导的中性粒细胞迁移中，与 LPS 暴露无关。
Kantrow SP, Shen Z, Jagneaux T, Zhang P, Nelson S. Neutrophil-mediated lung permeability and host defense proteins. Am J Physiol Lung Cell Mol Physiol 297: L738-L745, 2009. First published July 31, 2009; doi:10.1152/ajplung.00045.2009.-Neutrophil recruitment to the alveolar space is associated with increased epithelial permeability. The present study investigated in mice whether neutrophil recruitment to the lung leads to accumulation of plasma-derived host defense proteins in the alveolar space and whether respiratory burst contributes to this increase in permeability. Albumin, complement C1q, and IgM were increased in bronchoalveolar lavage (BAL) fluid 6 h after intratracheal LPS challenge. Neutrophil depletion before LPS treatment completely prevented this increase in BAL fluid protein concentration. Respiratory burst was not detected in neutrophils isolated from BAL fluid, and BAL proteins were increased in mice deficient in a key subunit of the respiratory burst apparatus, gp91(phox), similar to wild-type mice. Neutrophil recruitment elicited by intratracheal instillation of the chemokines macrophage inflammatory protein-2 and keratinocyte-derived chemokine was also accompanied by accumulation of albumin, C1q, and IgM. During neutrophil recruitment to the alveolar space, epithelial permeability facilitates delivery of host defense proteins. The observed increase in epithelial permeability requires recruitment of neutrophils, but not activation of the respiratory burst, and occurs with chemokine-induced neutrophil migration independent of LPS exposure.