Comparison of the effect of phenol and its derivatives on protein and free radical formation in human erythrocytes (in vitro)
Comparison of the effect of phenol and its derivatives on protein and free radical formation in human erythrocytes (in vitro)
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DOI：
10.1016/j.bcmd.2007.06.003
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发表时间：
2007-11-01
影响因子：
2.3
通讯作者：
Sicinska, P.
中科院分区：
文献类型：
--
作者：
Bukowska, B.;Michalowicz, J.;Sicinska, P.
研究了酚类化合物：苯酚、2,4-二氯苯酚 (2,4-DCP)、2,4-二甲基苯酚 (2,4-DMP) 和儿茶酚对人红细胞的影响。监测红细胞中酚类化合物氧化荧光标记6-羧基-2',7'-二氯二氢荧光素二乙酸酯(H(2)DCFDA)的水平以及羰基含量和血红蛋白变性。 H(2)DCFDA 已被广泛用作细胞水平氧化应激研究的标记物。我们注意到 2,4-DCP、2,4-DMP 和儿茶酚可诱导浓度和时间依赖性 H(2)DCFDA 氧化增加。我们还观察到与 2,4-DCP、儿茶酚和 2,4-DMP 一起孵育的红细胞中羰基含量增加以及参数 T（血红蛋白变性）的变化。最高水平的 H(2)DCFDA 氧化是由 2,4-DCP 引起的。红细胞中的蛋白质变化最大（以羰基含量测量）是由2,4-DMP引起的，但以参数T测量则由儿茶酚引起。据观察，孵育 3 小时后，苯酚不会将 H(2)DCFDA 氧化至浓度 2.5 mM。苯酚不会影响羰基含量，但会降低参数T（诱导血红蛋白变性）。综上所述，酚环中取代基的种类决定了单个化合物的分子作用机制和活性氧生成的能力，从而破坏了人红细胞的特定结构。 (C) 2007 Elsevier Inc. 保留所有权利。
The effect of phenolic compounds: phenol, 2,4-dichlorophenol (2,4-DCP), 2,4-dimethylphenol (2,4-DMP) and catechol on human erythrocytes was studied. The level of fluorescent label - 6-carboxy-2',7'-dichlorodihydrofluorescein diacetate (H(2)DCFDA) oxidation by phenolic compounds in erythrocytes as well as the carbonyl group content and hemoglobin denaturation were monitored. H(2)DCFDA has been utilized extensively as a marker for studies of oxidative stress at the cellular level.We noted that 2,4-DCP, 2,4-DMP and catechol induced an increase in the concentration- and time-dependent H(2)DCFDA oxidation. We also observed an increase in carbonyl group content and the changes in parameter T (denaturation of hemoglobin) in erythrocytes incubated with 2,4-DCP, catechol and 2,4-DMP. The highest level of H(2)DCFDA oxidation was provoked by 2,4-DCP. The biggest changes of proteins in erythrocytes measured as the carbonyl group content were induced by 2,4-DMP, but measured as parameter T they were induced by catechol. It was observed that phenol did not oxidize H(2)DCFDA up to the concentration of 2.5 mM after 3 h of incubation. Phenol did not affect the carbonyl group content but decreased parameter T (induced denaturation of hemoglobin).To sum up, the kind of the substituent in a phenolic ring determines the molecular mechanism of action of the individual compound and the capacity of reactive oxygen species generation and thus damages the specified structures in human erythrocytes. (C) 2007 Elsevier Inc. All rights reserved.