Localization of pulmonary surfactant protein during mouse lung development.
Localization of pulmonary surfactant protein during mouse lung development.
复制标题
小鼠肺发育过程中肺表面活性蛋白的定位。
DOI：
10.1016/0012-1606(84)90080-0
复制
发表时间：
1984
影响因子：
2.7
通讯作者：
Slavkin,HC
中科院分区：
文献类型：
--
作者：
Jaskoll,TF;Phelps,D;Taeusch,HW;Smith,BT;Slavkin,HC
在肺发育过程中，II 型肺泡上皮细胞产生细胞外肺表面活性剂。针对与人表面活性剂相关的非血清蛋白产生多克隆抗体。目前的研究旨在 (i) 确定小鼠表面活性剂蛋白是否与针对人表面活性剂蛋白的多克隆抗体发生抗原交叉反应； (ii) 确定胎儿、新生儿和成年小鼠肺部发育过程中表面活性蛋白的定位。二维凝胶电泳研究与免疫学技术相结合，提供了小鼠和人类表面活性蛋白共享抗原决定簇的证据。小鼠表面活性蛋白的主要单体形式在还原条件下是大约Mr35,000的糖蛋白。一种不太丰富的形式被鉴定为aMr45,000多肽。免疫组织化学定位显示，II 型细胞在 Theiler 26 期含有表面活性蛋白。免疫染色梯度位于肺泡表面内。在心脏、血管或肺间质细胞中未检测到抗原。在成熟成人肺的肺泡表面衬里检测到表面活性蛋白。这种蛋白质在胎儿和新生儿肺形态发生过程中的分布表明，肺表面活性剂的这种细胞外成分可能作为评估正常和异常肺发育的表型标记物非常有用。
During lung development type II alveolar epithelial cells produce extracellular pulmonary surfactant. Polyclonal antibodies were produced against nonserum proteins associated with human surfactant. The present studies were designed (i) to determine if mouse surfactant proteins were antigenically cross-reactive with polyclonal antibodies directed against human surfactant proteins; and (ii) to determine surfactant protein localization during fetal, neonatal, and adult mouse lung development. Two-dimensional gel electrophoresis studies in conjunction with immunologic techniques provided evidence that mouse and human surfactant proteins shared antigenic determinants. The major monomeric form of mouse surfactant protein is a glycoprotein of approximatelyMr35,000 under reducing conditions. A less abundant form was identified as aMr45,000 polypeptide. Immunohistochemical localization showed that type II cells contain surfactant protein at Theiler stage 26. A gradient of immunostaining was localized within alveolar surfaces. The antigen was not detected in heart, blood vessels, or pulmonary interstitial cells. Surfactant protein was detected lining alveolar surfaces in mature adult lung. The distribution of this protein during fetal and neonatal lung morphogenesis suggests that this extracellular constituent of pulmonary surfactant may be extremely useful as a phenotypic marker with which to evaluate normal and abnormal lung development.