Effects of low-level formaldehyde exposure on synaptic plasticity-related gene expression in the hippocampus of immunized mice
Effects of low-level formaldehyde exposure on synaptic plasticity-related gene expression in the hippocampus of immunized mice
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DOI：
10.1016/j.jneuroim.2007.03.010
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发表时间：
2007-05-01
影响因子：
3.3
通讯作者：
Fujimaki, Hidekazu
中科院分区：
文献类型：
--
作者：
Ahmed, Sohel;Tsukahara, Shinji;Fujimaki, Hidekazu
我们利用实时荧光定量PCR检测了吸入甲醛（FA, 400 ppb）对蛋清蛋白免疫小鼠海马n-甲基- d -天冬氨酸（NMDA）受体亚基（NR2A和NR2B）、多巴胺受体亚型（D1和D2）、环AMP反应元件结合蛋白(CREB)-1、CREB-2、FosB/Delta FosB和瞬时受体电位香草样受体（TRPV1）的影响。对pCREB进行Western blot分析。FA显著提高了NR2A、D1、D2受体和CREB-1 mRNA水平，但NR2B、CREB-2、FosB/Delta FosB和TRPV -1 mRNA水平不变。MK-801处理使FA诱导的mRNA水平正常化。FA暴露和MK-801处理对pCREB蛋白水平无显著影响。这些结果表明，FA暴露选择性上调免疫致敏小鼠海马基因表达。FA的作用可能是通过NMDA受体介导的谷氨酸能神经传递。(c) 2007 Elsevier B.V.版权所有
We examined the effects of inhalative exposure to formaldehyde (FA, 400 ppb) on N-methyl-D-aspartate (NMDA) receptor subunits (NR2A and NR2B), dopamine receptor subtypes (D1 and D2), cyclic AMP responsive element-binding protein (CREB)-1, CREB-2, FosB/Delta FosB, and transient receptor potential vanilloid receptor (TRPV1) in the hippocampus of ovalbumin-immunized mice using quantitative real-time PCR. Western blot analyses for pCREB were performed. The mRNA levels of NR2A, D1 and D2 receptors, and CREB-1 were significantly increased by FA, but NR2B, CREB-2, FosB/Delta FosB, and TRPV 1 mRNA levels remained unchanged. Treatment with MK-801 normalized the mRNA levels induced by FA. There was no significant effect of FA exposure and MK-801 treatment on the protein level of pCREB. These results indicate that FA exposure selectively up-regulates hippocampal gene expression in immunologically sensitized mice. The FA effects are presumably mediated by glutamatergic neurotransmission through NMDA receptors. (c) 2007 Elsevier B.V. All rights reserved.