A rapid method of non-destructive DNA extraction from individual springtails (Collembola)
A rapid method of non-destructive DNA extraction from individual springtails (Collembola)
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DOI：
10.1007/s13355-015-0340-0
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发表时间：
2015-08-01
影响因子：
1.3
通讯作者：
Nakamori, Taizo
中科院分区：
文献类型：
--
作者：
Aoyama, Hiroaki;Saitoh, Seikoh;Nakamori, Taizo
在这项研究中，我们描述了一种简单，快速的方法，非破坏性的DNA提取从一个单一的弹尾虫个体，没有解剖，裂解的标本，或提取的DNA纯化。我们证明，一个单一的标本已热处理后，在Tris-EDTA（TE）缓冲液中使用标准的热循环仪，该溶液可用于PCR扩增的线粒体细胞色素c氧化酶亚基1（COI）基因区，通常用于DNA条形码。该方法较好地保留了弹尾目中常用于物种鉴定的形态特征。这种DNA提取方法对于DNA条形码是优选的，其中需要测序和保存大量小的和易碎的标本。
In this study, we describe an easy and rapid method for non-destructive DNA extraction from a single Collembola individual, without dissection, lysis of the specimen, or purification of extracted DNA. We demonstrate that, after a single specimen has been heat-treated in Tris-EDTA (TE) buffer using a standard thermocycler, the solution can be used for PCR amplification of the mitochondrial cytochrome c oxidase subunit 1 (COI) gene region, typically used for DNA barcoding. With this method, the morphological features of Collembola commonly used for species identification are well preserved. This DNA extraction method is preferable for DNA barcoding where the sequencing and preservation of a large number of small and fragile specimens are required.