Taking advantage of rotifer sensitivity to rotenone to prevent pond crashes for algal-biofuel production
Taking advantage of rotifer sensitivity to rotenone to prevent pond crashes for algal-biofuel production
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DOI：
10.1016/j.algal.2015.03.013
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发表时间：
2015-07
影响因子：
5.1
通讯作者：
S. Ginkel;Thomas Igou;Zixuan Hu;A. Narode;Sarasija Cheruvu;Shusuke Doi;R. Johnston;T. Snell;Yongsheng Chen
中科院分区：
文献类型：
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作者：
S. Ginkel;Thomas Igou;Zixuan Hu;A. Narode;Sarasija Cheruvu;Shusuke Doi;R. Johnston;T. Snell;Yongsheng Chen
虽然藻类生物燃料具有许多优点，包括高面积生产力，但藻类可以被变形虫，纤毛虫和轮虫捕食，特别是在开放的池塘系统中。因此，需要控制这些藻类捕食者。本研究以小球藻为藻类，萼花臂尾轮虫为捕食者。农药鱼藤酮的效果进行了测试，目的是完全抑制轮虫，而只造成轻微的抑制。鱼藤酮24小时LC50。萼花为0.074 μM，而C.在0.8 μM浓度下，Kessler生长未受到抑制。鱼藤酮对Tetraselmissuecica种群增长的EC_（50）为0.56 μM，而对圆形臂尾轮虫和曼氏臂尾轮虫的EC_（50）分别为0.13和0.26 μM。轮虫的繁殖受到抑制，甚至在较低的鱼藤酮浓度比那些导致死亡。在共培养中，鱼藤酮浓度为0.1 μM时，几乎完全引起B。萼花死亡率C.凯斯勒生长不受影响。应用鱼藤酮在一年内生产100万加仑生物柴油的成本估计为842美元。这为藻类养殖者提供了一种重要且廉价的工具，以管理藻类大量养殖中的掠食者并避免池塘崩溃。
Although algae-biofuels have many advantages including high areal productivity, algae can be preyed upon by amoeba, ciliates, and rotifers, particularly in open pond systems. Thus, these algal predators need to be controlled. In this study,Chlorellakessleriwas used as the algae and the freshwater rotifer Brachionuscalyciflorusas the predator. The effect of the pesticide, rotenone, was tested with the goal of totally inhibiting the rotifer while causing only minor inhibition to the alga. The rotenone 24-hr LC50forB. calycifloruswas 0.074 μM whileC. kesslerigrowth was uninhibited at 0.8 μM. The rotenone EC50for population growth of the marine algaTetraselmissuecicawas 0.56 μM, in contrast to 0.13 and 0.26 μM for the marine rotifersBrachionus rotundiformisandBrachionus manjavacas, respectively. Rotifer reproduction was inhibited at even lower rotenone concentrations than those causing mortality. In co-culture, a rotenone concentration of 0.1 μM caused near completeB. calyciflorusmortality while leavingC. kesslerigrowth unaffected. Rotenone applications to produce 1 million gallons of biodiesel in one year are estimated to cost $842. This provides algae farmers an important and inexpensive tool to manage grazing predators in algae mass cultures and avoid pond crashes.