Sequence and analysis of the genome of a Baculovirus pathogenic for Lymantria dispar
Sequence and analysis of the genome of a Baculovirus pathogenic for Lymantria dispar
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DOI：
10.1006/viro.1998.9469
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发表时间：
1999-01-05
期刊：
影响因子：
3.7
通讯作者：
Rohrmann, GF
中科院分区：
文献类型：
--
作者：
Kuzio, J;Pearson, MN;Rohrmann, GF
对舞毒蛾核型多角体病毒（LdMNPV）的全基因组进行了序列测定和分析。它由161，046个碱基组成，G + C含量为57.5%，含有163个大于或等于150个核苷酸的推定开放阅读框架（ORF）。在苜蓿银纹夜蛾核型多角体病毒（Autographa californica MNPV，AcMNPV）基因组预测的155个基因中，有95个基因具有同源性。LdMNPV基因组的9%以上被16个与AcMNPV ORF 2相关的重复基因所占据。已经报道在AcMNPV生命周期中发挥重要作用的几个基因的容易识别的同源物不存在;这些包括ie-2，转录反式激活因子，和gp 64，病毒的非封闭形式的主要包膜糖蛋白。鉴定了AcMNPV中缺乏但存在于其他杆状病毒中的许多基因;这些基因包括两个病毒增强因子同源物、芋螺毒素样基因的第二个拷贝和dutpase同源物。尽管发现了预测编码核糖核苷酸还原酶大亚基的单个基因，但存在小亚基基因的两个不同拷贝。此外，同源基因以前没有报告的杆状病毒进行了鉴定，包括一个预测的蛋白质与DNA连接酶的同源性和另一个具有最密切相关的酵母线粒体解旋酶的基序。13个同源区域（hrs）含有54个重复序列，其中包括30 bp的不完全回文序列进行了鉴定。不完全回文酶与其他杆状病毒的回文酶相关。(C)北京：科学出版社.
The genome of the Lymantria dispar multinucleocapsid nucleopolyhedrovirus (LdMNPV) was sequenced and analyzed. It is composed of 161,046 bases with a G + C content of 57.5% and contains 163 putative open reading frames (ORFs) of greater than or equal to 150 nucleotides. Homologs were found to 95 of the 155 genes predicted for the Autographa californica MNPV (AcMNPV) genome. More than 9% of the LdMNPV genome was occupied by 16 repeated genes related to AcMNPV ORF2. Readily identifiable homologs of several genes that have been reported to play important roles in the AcMNPV life cycle are not present; these include ie-2, a transcriptional transactivator, and gp64, a major envelope glycoprotein of the nonoccluded form of the virus. A number of genes lacking in AcMNPV but present in other baculoviruses were identified; these include two viral enhancing factor homologs, a second copy of a conotoxin-like gene, and a dutpase homolog. Although a single gene predicted to encode a large subunit of ribonucleotide reductase was found, two different copies of the small subunit gene were present. In addition, homologs of genes not previously reported for baculoviruses were identified, including a predicted protein with homology to DNA ligases and another that has motifs most closely related to a yeast mitochondrial helicase. Thirteen homologous regions (hrs) containing 54 repeated sequences that include 30-bp imperfect palindromes were identified. The imperfect palindromes are related to those from other baculoviruses. (C) 1999 Academic Press.