RECD - THE GENE FOR AN ESSENTIAL 3RD SUBUNIT OF EXONUCLEASE-V
RECD - THE GENE FOR AN ESSENTIAL 3RD SUBUNIT OF EXONUCLEASE-V
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DOI：
10.1073/pnas.83.15.5558
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发表时间：
1986-08-01
影响因子：
11.1
通讯作者：
SMITH, GR
中科院分区：
文献类型：
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作者：
AMUNDSEN, SK;TAYLOR, AF;SMITH, GR
大肠杆菌的核酸外切酶V（EC 3.1.11.5）是一种具有促进基因重组的多种活性的酶，先前已显示含有两种多肽，即recB和recC基因的产物。我们在此报道，该酶还含有第三多肽（α）。分子量约为58 kDa。α多肽不由一类突变体（先前命名为recB.dbldag.）缺乏核酸外切酶V的核酸酶活性但保留重组能力。基因recD，编码α.多肽在E上以thyA-recC-ptr-recB-recD-argA的顺序位于recB附近。coli染色体。recB和recD基因似乎由recB左侧的共同启动子控制;一个较弱的启动子似乎单独控制recD。根据这些结果，我们讨论了外切核酸酶V的三种多肽的结构和功能之间的关系，在此也称为RecBCD酶。
Exonuclease V (EC 3.1.11.5) of Escherichia coli, an enzyme with multiple activities promoting genetic recombination, has previously been shown to contain two polypeptides, the products of the recB and recC genes. We report here that the enzyme contains in addition a third polypeptide (.alpha.) with a molecular mass of about 58 kDa. The .alpha. polypeptide is not synthesized by a class of mutants (previously designated recB.dbldag.) lacking the nuclease activity of exonuclease V but retaining recombination proficiency. The gene, recD, coding for the .alpha. polypeptide is located near recB in the order thyA-recC-ptr-recB-recD-argA on the E. coli chromosome. The recB and recD genes appear to be governed by a common promoter of the left of recB; a weaker promoter appears to govern recD alone. In the light of these results we discuss the relation between the structure and function of the three polypeptides of exonuclease V, hereby alternatively designated RecBCD enzyme.